Invitrogen

Ankyrin B Monoclonal Antibody (N105/13), PerCP

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-45528

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

1:200
-

Immunocytochemistry (ICC/IF)

1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N105/13

Immunogen

Synthetic peptide amino acids 203-496 of human Ankyrin-B
View immunogen

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931982

Product Specific Information

1 µg/mL of MA5-45528 was sufficient for detection of Ankyrin-B in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 200kDa. No cross reactivity against Ankyrin-G.

This antibody was formerly sold as clone S105-13.

Target Information

This gene encodes a member of the ankyrin family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4. Multiple transcript variants encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ANK-2; ank2; ankyrin 2, brain; ankyrin 3, epithelial; ankyrin B; ankyrin, brain; Ankyrin-2; ankyrin-2, nonerythrocytic; Ankyrin-B; Brain ankyrin; Non-erythroid ankyrin

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Gene Aliases: AI835472; ANK-2; ANK2; Ank3; ANKB; AW491075; brank-2; LQT4

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UniProt ID: (Human) Q01484, (Mouse) Q8C8R3

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Entrez Gene ID: (Human) 287, (Mouse) 109676, (Rat) 362036

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Function(s)
structural constituent of cytoskeleton protein binding cytoskeletal adaptor activity enzyme binding protein kinase binding spectrin binding protein binding, bridging ion channel binding ATPase binding potassium channel regulator activity
Process(es)
regulation of heart rate cellular calcium ion homeostasis signal transduction protein localization positive regulation of gene expression regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of cardiac muscle contraction by calcium ion signaling regulation of protein stability T-tubule organization protein localization to cell surface cellular protein localization protein localization to M-band protein localization to T-tubule regulation of apoptotic process positive regulation of potassium ion transport protein stabilization regulation of calcium ion transport positive regulation of calcium ion transport regulation of cardiac muscle contraction cardiac muscle contraction protein localization to endoplasmic reticulum protein localization to plasma membrane protein targeting to plasma membrane regulation of cardiac muscle cell contraction atrial cardiac muscle cell action potential SA node cell action potential regulation of cardiac muscle cell membrane potential atrial cardiac muscle cell to AV node cell communication SA node cell to atrial cardiac muscle cell communication regulation of heart rate by cardiac conduction regulation of AV node cell action potential regulation of SA node cell action potential regulation of atrial cardiac muscle cell action potential positive regulation of potassium ion transmembrane transporter activity regulation of calcium ion transmembrane transporter activity positive regulation of calcium ion transmembrane transporter activity regulation of cation channel activity positive regulation of cation channel activity atrial septum development ER to Golgi vesicle-mediated transport cytoskeleton organization paranodal junction assembly protein localization to organelle regulation of release of sequestered calcium ion into cytosol response to methylmercury regulation of ventricular cardiac muscle cell membrane repolarization sarcoplasmic reticulum calcium ion transport ventricular cardiac muscle cell action potential membrane depolarization during SA node cell action potential nervous system development
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