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          • Primary Antibodies ›
          • Ankyrin G Antibodies

          Invitrogen

          Ankyrin G Monoclonal Antibody (4G3F8)

          Advanced Verification
          26 Published Figures
          37 References
          View all (14) Ankyrin G antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Ankyrin G Monoclonal Antibody (4G3F8)

          • Antibody Testing Data (3)
          • Published Figures (26)
          • Advanced Verification (1)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 30

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          Ankyrin G Antibody (33-8800) in ICC/IF

          Immunofluorescence analysis of Ankyrin G was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Ankyrin G Monoclonal Antibody (4G3F8) (Product # 33-8800) at 5 µg/mL in 0.1% BSA, incubated at 4 degree ce... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Western Blot (WB)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunocytochemistry (ICC/IF)
          Ankyrin G Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Ankyrin G Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Ankyrin G Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Ankyrin G Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Ankyrin G Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Ankyrin G Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Ankyrin G Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Ankyrin G Antibody in Immunohistochemistry (IHC)
          Ankyrin G Antibody in Immunohistochemistry (IHC)
          Ankyrin G Antibody in Immunohistochemistry (IHC)
          Ankyrin G Antibody in Western Blot (WB)
          Ankyrin G Antibody

          Product Details

          33-8800

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 10 publications 10 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 4 publications 4 publications

          Immunocytochemistry (ICC/IF)

          2-5 µg/mL
          View 18 publications 18 publications

          ELISA (ELISA)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -

          in situ PLA (PLA)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Rabbit, Rat

          Published species

          Bovine, Dog, Fruit fly, Hamster, Human, Mouse, Pig, Rat, Sheep

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          4G3F8

          Immunogen

          A synthetic peptide derived from the spectrin-binding domain of the human ankyrin G protein.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533145

          Target Information

          Ankyrin-G is found in several tissues including brain where, due to tissue-specific RNA processing, it is expressed as two different isoforms, localized to axon initial segments and nodes of Ranvier. Ankyrin-G is also found at sites of epithelial cell-cell contact and in the intracellular membranes of some cells. Ankyrin-G isoforms contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following a globular head domain. Ankyrin-G is thought to participate in the maintenance and targeting of ion channels and cell adhesion molecules to the nodes of Ranvier and to axon initial segments.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ANK-3; ankyrin 3 (G); ankyrin 3, epithelial; Ankyrin-3; Ankyrin-G; brain-specific ankyrin-G

          View more View less

          Gene Aliases: 2900054D09Rik; AI314020; ANK-3; ANK3; AnkG; Ankyrin-3; ANKYRIN-G; MRT37

          View more View less

          UniProt ID: (Human) Q12955, (Rat) O70511, (Mouse) Q61308

          View more View less

          Entrez Gene ID: (Human) 288, (Dog) 479215, (Rat) 361833, (Mouse) 11735

          View more View less

          Function(s)
          structural constituent of cytoskeleton protein binding cytoskeletal protein binding cytoskeletal adaptor activity spectrin binding protein binding, bridging ion channel binding cadherin binding
          Process(es)
          mitotic cytokinesis plasma membrane organization signal transduction axonogenesis axon guidance neuromuscular junction development positive regulation of gene expression positive regulation of cell communication by electrical coupling positive regulation of sodium ion transport magnesium ion homeostasis neuronal action potential positive regulation of homotypic cell-cell adhesion Golgi to plasma membrane protein transport regulation of potassium ion transport establishment of protein localization positive regulation of membrane potential synapse organization cellular response to magnesium ion membrane assembly protein localization to plasma membrane maintenance of protein location in plasma membrane protein targeting to plasma membrane positive regulation of protein targeting to membrane positive regulation of membrane depolarization during cardiac muscle cell action potential negative regulation of delayed rectifier potassium channel activity positive regulation of sodium ion transmembrane transporter activity positive regulation of cation channel activity ER to Golgi vesicle-mediated transport cytoskeletal anchoring at plasma membrane positive regulation of action potential
          It has to be done as per old AB suggested Products section.
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