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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Annexin A1 Antibodies

          Invitrogen

          Annexin A1 Polyclonal Antibody

          Advanced Verification
          34 Published Figures
          51 References
          View all (74) Annexin A1 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Annexin A1 Polyclonal Antibody

          • Antibody Testing Data (8)
          • Published Figures (34)
          • Advanced Verification (2)
          Annexin A1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Annexin A1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 44

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          Annexin A1 Antibody (71-3400) in ICC/IF

          Immunofluorescent analysis of Annexin-1 Antibody was done on 70% confluent log phase U87-MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Annexin-1 Antibody (Product # 71-3400) at 1:1000 dilution in 1% BSA and incubated for 3 hours at room temperat... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Annexin A1 Antibody in Immunocytochemistry (ICC/IF)
          Annexin A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Annexin A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Annexin A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Annexin A1 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
          Annexin A1 Antibody in Immunocytochemistry (ICC/IF)
          Annexin A1 Antibody in Immunocytochemistry (ICC/IF)
          Annexin A1 Antibody in Immunocytochemistry (ICC/IF)
          Annexin A1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Annexin A1 Antibody in Immunocytochemistry (ICC/IF)
          Annexin A1 Antibody in Immunohistochemistry (IHC)
          Annexin A1 Antibody in Immunohistochemistry (IHC)
          Annexin A1 Antibody in Immunohistochemistry (IHC)
          Annexin A1 Antibody in Immunohistochemistry (IHC)
          Annexin A1 Antibody in Immunohistochemistry (IHC)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Western Blot (WB)
          Annexin A1 Antibody in Flow Cytometry (Flow)
          Annexin A1 Antibody in Flow Cytometry (Flow)
          Annexin A1 Antibody in ELISA (ELISA)
          Annexin A1 Antibody
          Annexin A1 Antibody

          Product Details

          71-3400

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:100-1:1,000
          View 24 publications 24 publications

          Immunohistochemistry (IHC)

          -
          View 10 publications 10 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1 µg/mL
          View 9 publications 9 publications

          Flow Cytometry (Flow)

          -
          View 4 publications 4 publications

          ELISA (ELISA)

          1:500-1:50,000
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Full-length human recombinant Annexin I protein purified from yeast.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          whole serum

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533983

          Product Specific Information

          This antibody is specific for the Annexin I protein and does not cross-react with other Annexins.Reactivity with Annexin I was confirmed by western blotting using total lysates derived from human fibroblasts. Positive control lysates used: human fibroblasts, mouse kidney, mouse lung, adult rat and mouse brain.

          Target Information

          Annexin I belongs to a family of Ca (2+) -dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. Annexin I has a molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ANAX1; Annexin 1 (p35) (Lipocortin 1); Annexin A1; Annexin I; annexin I (lipocortin I); Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; lipocortin 1; Lipocortin I; p35; Phospholipase A2 inhibitory protein; RP11-71A24.1

          View more View less

          Gene Aliases: Anx-1; Anx-A1; ANX1; ANXA1; C430014K04Rik; Lpc-1; LPC1; p35

          View more View less

          UniProt ID: (Human) P04083, (Mouse) P10107, (Rat) P07150

          View more View less

          Entrez Gene ID: (Human) 301, (Mouse) 16952, (Rat) 25380

          View more View less

          Function(s)
          single-stranded DNA binding single-stranded RNA binding helicase activity receptor binding structural molecule activity calcium ion binding protein binding phospholipid binding calcium-dependent phospholipid binding phospholipase A2 inhibitor activity protein binding, bridging double-stranded DNA-dependent ATPase activity annealing helicase activity protein homodimerization activity calcium-dependent protein binding cadherin binding involved in cell-cell adhesion phospholipase inhibitor activity
          Process(es)
          DNA strand renaturation neutrophil homeostasis signal transduction cell surface receptor signaling pathway peptide cross-linking insulin secretion keratinocyte differentiation positive regulation of vesicle fusion positive regulation of prostaglandin biosynthetic process DNA duplex unwinding positive regulation of neutrophil apoptotic process DNA rewinding regulation of cell proliferation positive regulation of apoptotic process alpha-beta T cell differentiation arachidonic acid secretion negative regulation of protein secretion cellular response to hydrogen peroxide prolactin secretion cellular response to glucocorticoid stimulus neutrophil clearance positive regulation of G1/S transition of mitotic cell cycle negative regulation of phospholipase A2 activity negative regulation of interleukin-8 secretion response to hormone response to X-ray response to organic cyclic compound prostate gland development endocrine pancreas development response to corticosteroid response to estradiol gliogenesis response to drug response to peptide hormone estrous cycle response to glucocorticoid hepatocyte differentiation response to interleukin-1 adaptive immune response monocyte chemotaxis regulation of leukocyte migration phagocytosis inflammatory response G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger regulation of cell shape myoblast migration involved in skeletal muscle regeneration actin cytoskeleton reorganization regulation of interleukin-1 production positive regulation of interleukin-2 production positive regulation of T cell proliferation negative regulation of apoptotic process innate immune response positive regulation of T-helper 1 cell differentiation negative regulation of T-helper 2 cell differentiation negative regulation of exocytosis regulation of hormone secretion regulation of inflammatory response granulocyte chemotaxis positive regulation of wound healing
          It has to be done as per old AB suggested Products section.
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