Invitrogen

Annexin A2 Monoclonal Antibody (Z014)

Annexin A2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

Annexin A2 Antibody (03-4400) in ICC/IF

Immunofluorescence analysis of Annexin A2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 15 minutes at room temperature. The cells were labeled with Annexin A2 Monoclonal Antibody (Z014) (Product # 03-4400) at 5 µg in 0.1% BSA, incubated at 4 degree celsi... View More

Annexin A2 Antibody in Western Blot (WB)

Annexin A2 Antibody in Neutralization (Neu)

Product Image Gallery

Product Details

03-4400

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

5-10 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

0.1-1.0 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Dog, Human, Sheep

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Z014

Immunogen

Annexin II purified from bovine lung.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

purified

Storage buffer

proprietary buffer

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2532925

Target Information

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

METTL3-mediated ANXA2 inhibition confers neuroprotection in ischemic stroke: Evidence from in vivo and in vitro studies.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liu B,Xing Z,Song F,Li D,Zhao B,Xu C,Xia W,Ji H

03-4400 was used in Flow Cytometry to reveal the neuroprotective effects of METTL3-mediated ANXA2/p38MAPK/MMP-9 inhibition against ischemic stroke.

Sat Jul 01 00:00:00 UTC 2023

Annexin A2 Enhances Complement Activation by Inhibiting Factor H.

Journal of immunology (Baltimore, Md. : 1950)

Renner B,Tong HH,Laskowski J,Jonscher K,Goetz L,Woolaver R,Hannan J,Li YX,Hourcade D,Pickering MC,Holers VM,Thurman JM

34400 was used in flow cytometry and immunohistochemistry - frozen section to identify annexin A2 as a binding partner of factor H

Mon Feb 01 00:00:00 UTC 2016

Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker.

International journal for parasitology

Yonglitthipagon P,Pairojkul C,Chamgramol Y,Mulvenna J,Sripa B

03-4400 was used in Western Blotting to study how ANXA2 expression in tumors may be useful for predicting the poor outcome of CCA patients.

Sun Aug 15 00:00:00 UTC 2010

Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker.

International journal for parasitology

Yonglitthipagon P,Pairojkul C,Chamgramol Y,Mulvenna J,Sripa B

03-4400 was used in Western Blotting to study how ANXA2 expression in tumors may be useful for predicting the poor outcome of CCA patients.

Sun Aug 15 00:00:00 UTC 2010

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

The Journal of biological chemistry

Hastie C,Masters JR,Moss SE,Naaby-Hansen S

03-4400 was used in blocking or activating experiment to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells

Fri May 02 00:00:00 UTC 2008

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

The Journal of biological chemistry

Hastie C,Masters JR,Moss SE,Naaby-Hansen S

03-4400 was used in blocking or activating experiment to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells

Fri May 02 00:00:00 UTC 2008

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

The Journal of biological chemistry

Hastie C,Masters JR,Moss SE,Naaby-Hansen S

03-4400 was used in blocking or activating experiment to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells

Fri May 02 00:00:00 UTC 2008

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

The Journal of biological chemistry

Hastie C,Masters JR,Moss SE,Naaby-Hansen S

03-4400 was used in blocking or activating experiment to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells

Fri May 02 00:00:00 UTC 2008

Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells.

The Journal of biological chemistry

Hastie C,Masters JR,Moss SE,Naaby-Hansen S

03-4400 was used in blocking or activating experiment to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells

Fri May 02 00:00:00 UTC 2008

Involvement of annexin A2 in anti-beta2GPI/beta2GPI-induced tissue factor expression on monocytes.

Cell research

Zhou H,Ling S,Yu Y,Wang T,Hu H

03-4400 was used in Western Blotting to show that annexin 2 is expressed on monocytes, and is involved in monocyte activation and tissue factor induced by anti-β2GPI/β2GPI.

Wed Aug 01 00:00:00 UTC 2007

Annexin II mediates plasminogen-dependent matrix invasion by human monocytes: enhanced expression by macrophages.

Blood

Brownstein C,Deora AB,Jacovina AT,Weintraub R,Gertler M,Khan KM,Falcone DJ,Hajjar KA

Thu Jan 01 00:00:00 UTC 2004

An annexin 2 phosphorylation switch mediates p11-dependent translocation of annexin 2 to the cell surface.

The Journal of biological chemistry

Deora AB,Kreitzer G,Jacovina AT,Hajjar KA

Fri Oct 15 00:00:00 UTC 2004

An annexin 2 phosphorylation switch mediates p11-dependent translocation of annexin 2 to the cell surface.

The Journal of biological chemistry

Deora AB,Kreitzer G,Jacovina AT,Hajjar KA

Fri Oct 15 00:00:00 UTC 2004

Integrin alphaMbeta2 orchestrates and accelerates plasminogen activation and fibrinolysis by neutrophils.

The Journal of biological chemistry

Pluskota E,Soloviev DA,Bdeir K,Cines DB,Plow EF

03-4400 was used in blocking or activating experiment to show that the alpha(M)beta(2) regulates neutrophil-dependent fibrinolysis

Fri Apr 23 00:00:00 UTC 2004

Inactivation of annexin II tetramer by S-nitrosoglutathione.

European journal of biochemistry

Liu L,Enright E,Sun P,Tsai SY,Mehta P,Beckman DL,Terrian DM

Sun Sep 01 00:00:00 UTC 2002

Inactivation of annexin II tetramer by S-nitrosoglutathione.

European journal of biochemistry

Liu L,Enright E,Sun P,Tsai SY,Mehta P,Beckman DL,Terrian DM

Sun Sep 01 00:00:00 UTC 2002

Suppressive effect on lymphoproliferation in vitro by soluble annexin II released from isolated placental membranes.

American journal of reproductive immunology (New York, N.Y. : 1989)

Aarli A,Kristoffersen EK,Jensen TS,Ulvestad E,Matre R

03-4400 was used in western blot to test if annexin II is released from syncytiotrophoblast microvillous plasma membranes and if it has any immunosuppressive effect.

Sat Nov 01 00:00:00 UTC 1997

Bioinformatics

Protein Aliases: annexin 2; Annexin A2; Annexin II; Annexin-2; Calpactin I heavy chain; calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; Chromobindin-8; epididymis secretory protein Li 270; Lipocortin II; p36; PAP-IV; Placental anticoagulant protein IV; Protein I

View more View less

Gene Aliases: ANX2; ANX2L4; ANXA2; BOS_10772; CAL1H; HEL-S-270; LIP2; LPC2; LPC2D; P36; PAP-IV

View more View less

UniProt ID: (Human) P07355, (Bovine) P04272, (Dog) Q6TEQ7, (Sheep) A2SW69

View more View less

Entrez Gene ID: (Human) 302, (Bovine) 282689, (Dog) 403435, (Sheep) 100048993

View more View less

Function(s)

protease binding calcium ion binding protein binding calcium-dependent phospholipid binding phosphatidylinositol-4,5-bisphosphate binding cytoskeletal protein binding Rab GTPase binding phospholipase A2 inhibitor activity receptor activator activity S100 protein binding poly(A) RNA binding calcium-dependent protein binding cadherin binding involved in cell-cell adhesion phospholipase inhibitor activity

Process(es)

angiogenesis membrane raft assembly positive regulation of protein phosphorylation negative regulation of receptor internalization membrane budding body fluid secretion collagen fibril organization positive regulation of vesicle fusion negative regulation of low-density lipoprotein particle receptor catabolic process osteoclast development fibrinolysis negative regulation of catalytic activity positive regulation of fibroblast proliferation positive regulation of binding protein heterotetramerization protein targeting to plasma membrane response to thyroid hormone cell-cell adhesion negative regulation of receptor binding positive regulation of receptor activity cellular response to acid chemical

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Annexin A2 Monoclonal Antibody (Z014)

Annexin A2 Antibody (03-4400) in ICC/IF

Product Details

03-4400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!