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Invitrogen
Annexin A6 Monoclonal Antibody (4C4)
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FIGURE: 1 / 9
Annexin A6 Antibody (MA5-49264) in ICC/IF
Immunocytochemistry analysis of Annexin A6 in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Annexin A6 Monoclonal antibody (Product # MA5-49264) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and inc... View More
Product Details
MA5-49264
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
4C4
Immunogen
E. coli-derived human Annexin VI recombinant protein (Position: N395-L665).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074922
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human plancenta tissue, human U87 whole cell, human HepG2 whole cell, human Hela whole cell, rat thymus tissue, rat pancreas tissue, mouse thymus tissue, mouse pancreas tissue. IHC: human liver tissue, human lung cancer tissue, human renal clear cell carcinoma tissue, mouse pancreas tissue, rat pancreas tissue. ICC/IF: T-47D cell. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Exon 21 of annexin VI is alternatively spliced, giving rise to two isoforms that differ by a 6-amino acid insertion at the start of the seventh repeat. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 67 kDa calelectrin; Annexin A6; Annexin VI; annexin VI (p68); annexin VI, p68; Annexin-6; calcium-binding protein 65/67; calcium-binding protein CATA 65/67; calcium-binding protein p68; calelectrin; calphobindin II; Calphobindin-II; CBP 65/67; Chromobindin-20; CPB-II; Lipocortin VI; p68; p70; Protein III; testis secretory sperm-binding protein Li 198a
Gene Aliases: ANX6; ANXA6; AnxVI; AW107198; Cabm; Camb; CBP68; CPB-II
UniProt ID: (Human) P08133, (Mouse) P14824
Entrez Gene ID: (Human) 309, (Mouse) 11749, (Rat) 79125
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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