Invitrogen

ApoA1 Monoclonal Antibody (311)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MIA1402

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

ELISA (ELISA)

1 µg/mL
View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent
-

Functional Assay (Functional)

-
View 1 publication 1 publication

Radioimmune Assays (RIA)

Assay-dependent
-

In vitro Assay (IV)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

311

Immunogen

Purified human plasma Apo A-1
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152130

Product Specific Information

By sandwich ELISA, MIA1402 can be used as a detection antibody with Product # 710263 or # 701239 as a coating antibody, to generate a matched pair. Using these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. MIA1402 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1402, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.

By Western blot, MIA1402 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1402 may not be as successful for detecting endogenous Apo A-1 by Western blot.

MIA1402 was formerly sold as a Seradyn product.

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 1-Sep; 2-Sep; Apo a; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; Udpgt; Ugt1; Ugt1a7

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Gene Aliases: Alp-1; apo(a); apo-AI; Apoa-1; apoA-I; APOA1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2

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UniProt ID: (Human) P02647, (Mouse) Q00623

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Entrez Gene ID: (Human) 335, (Mouse) 11806

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Function(s)
beta-amyloid binding protein binding phospholipid binding phospholipid transporter activity high-density lipoprotein particle binding cholesterol binding cholesterol transporter activity enzyme binding phosphatidylcholine binding apolipoprotein receptor binding apolipoprotein A-I receptor binding identical protein binding chemorepellent activity lipase inhibitor activity phosphatidylcholine-sterol O-acyltransferase activator activity high-density lipoprotein particle receptor binding lipid transporter activity lipid binding lipoprotein particle binding
Process(es)
regulation of protein phosphorylation endothelial cell proliferation negative regulation of cytokine secretion involved in immune response lipid metabolic process phospholipid metabolic process phosphatidylcholine biosynthetic process cholesterol biosynthetic process transport lipid transport G-protein coupled receptor signaling pathway integrin-mediated signaling pathway protein localization steroid metabolic process cholesterol metabolic process glucocorticoid metabolic process negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol esterification positive regulation of triglyceride catabolic process negative regulation of very-low-density lipoprotein particle remodeling phospholipid transport protein oxidation peptidyl-methionine modification triglyceride catabolic process lipid storage regulation of intestinal cholesterol absorption cholesterol transport adrenal gland development organ regeneration axon regeneration regulation of Cdc42 protein signal transduction cholesterol efflux phospholipid efflux negative regulation of heterotypic cell-cell adhesion very-low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly positive regulation of Rho protein signal transduction lipoprotein metabolic process lipoprotein biosynthetic process cholesterol homeostasis blood vessel endothelial cell migration reverse cholesterol transport positive regulation of fatty acid biosynthetic process phosphatidylcholine metabolic process negative regulation of interleukin-1 beta secretion negative regulation of inflammatory response protein stabilization negative chemotaxis positive regulation of lipoprotein lipase activity vitamin transport positive regulation of hydrolase activity negative regulation of hydrolase activity positive regulation of transferase activity positive regulation of stress fiber assembly phospholipid homeostasis negative regulation of lipase activity negative regulation of cell adhesion molecule production negative regulation of response to cytokine stimulus triglyceride homeostasis cholesterol import positive regulation of substrate adhesion-dependent cell spreading retinoid metabolic process platelet degranulation receptor-mediated endocytosis transforming growth factor beta receptor signaling pathway response to nutrient peripheral nervous system axon regeneration neuron projection regeneration high-density lipoprotein particle clearance response to drug response to estrogen cellular protein metabolic process transmembrane transport ERK1 and ERK2 cascade
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