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Invitrogen
ApoA2 Recombinant Rabbit Monoclonal Antibody (43H22L4)
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FIGURE: 1 / 6
ApoA2 Antibody (701236) in ICC/IF
Immunofluorescence analysis of Apolipoprotein A-II was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ApoA2 Recombinant Rabbit Monoclonal Antibody (43H22L4) (Product # 701236) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, A... View More
Product Details
701236
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
43H22L4
Immunogen
Peptide corresponding to amino acids 60-71 of human Apo A2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532435
Product Specific Information
This antibody is predicted to react with non-human primate and mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL, and VLDL particles of human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins ( especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease that measurements of lipoprotein particles (especially HDL and LDL). The apolipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APO A2; Apo-AII; Apolipoprotein; Apolipoprotein A-II; Apolipoprotein A2; Apolipoprotein AII
Gene Aliases: Apo-AII; ApoA-II; APOA2; APOAII
UniProt ID: (Human) B2R524, (Rat) P04638
Entrez Gene ID: (Human) 336, (Rat) 25649
lipid transporter activity
protein binding
phospholipid binding
high-density lipoprotein particle binding
lipid binding
cholesterol binding
cholesterol transporter activity
phosphatidylcholine binding
apolipoprotein receptor binding
protein homodimerization activity
protein heterodimerization activity
lipase inhibitor activity
phosphatidylcholine-sterol O-acyltransferase activator activity
high-density lipoprotein particle receptor binding
acute inflammatory response
negative regulation of cytokine secretion involved in immune response
protein folding
phosphatidylcholine biosynthetic process
lipid transport
cholesterol metabolic process
phospholipid catabolic process
response to glucose
positive regulation of cholesterol esterification
negative regulation of very-low-density lipoprotein particle remodeling
protein oxidation
peptidyl-methionine modification
regulation of intestinal cholesterol absorption
cholesterol transport
organ regeneration
regulation of protein stability
negative regulation of cholesterol transport
cholesterol efflux
phospholipid efflux
triglyceride-rich lipoprotein particle remodeling
low-density lipoprotein particle remodeling
high-density lipoprotein particle remodeling
high-density lipoprotein particle assembly
high-density lipoprotein particle clearance
lipoprotein metabolic process
response to drug
cholesterol homeostasis
positive regulation of catalytic activity
response to estrogen
reverse cholesterol transport
positive regulation of interleukin-8 biosynthetic process
diacylglycerol catabolic process
negative regulation of lipid catabolic process
positive regulation of lipid catabolic process
response to glucocorticoid
negative regulation of lipase activity
negative regulation of cholesterol import
negative regulation of cholesterol transporter activity
retinoid metabolic process
triglyceride metabolic process
viral process
lipoprotein biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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