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Invitrogen
Apolipoprotein B Recombinant Rabbit Monoclonal Antibody (2T5X0)
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Apolipoprotein B Antibody (MA5-35458) in WB
Western blot analysis of Apolipoprotein B in extracts of various cell lines. Samples were incubated with Apolipoprotein B Monoclonal antibody (Product # MA5-35458) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
Product Details
MA5-35458
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2T5X0
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ApoB (P04114)
Conjugate
Form
Liquid
Concentration
0.67 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849359
Product Specific Information
Immunogen sequence: RKYTYNYEAE SSSGVPGTAD SRSATRINCK VELEVPQLCS FILKTSQCTL KEVYGFNPEG KALLKKTKNS EEFAAAMSRY ELKLAIPEGK QVFLYPEKDE P
Target Information
Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL) with a molecular mass of approximately 260-500 kDa. Apolipoprotein B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation. Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearance of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. MDA-oxidized LDL appear to be different from LDL oxidized by other means. Apolipoprotein B is mostly synthesized in the liver, and is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L. Apolipoprotein B occurs in plasma as two main isoforms, apoB-48 and apoB-100. The intestinal and the hepatic forms of Apolipoprotein B are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in the Apolipoprotein B gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective Apolipoprotein B, diseases affecting plasma cholesterol and Apolipoprotein B levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apo B-100; Apo B100; apo-B; ApoB 100; ApoB 48; apolipo b; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B 100; Apolipoprotein B 48; apolipoprotein B PI; Apolipoprotein B-100; apolipoprotein B-48; Apolipoprotein B100; apolipoprotein B46; apolipoprotein B47; apolipoprotein B49; LOX-1; mCG_129875; MGC176318; Ox-LDL receptor 1
Gene Aliases: Aa1064; Ac1-060; AI315052; Apo B-100; Apob; ApoB-100; ApoB-48
UniProt ID: (Mouse) E9Q414, (Rat) Q7TMA5
Entrez Gene ID: (Mouse) 238055, (Rat) 54225
in utero embryonic development
lipid metabolic process
triglyceride mobilization
transport
lipid transport
spermatogenesis
nervous system development
steroid metabolic process
cholesterol metabolic process
fertilization
response to carbohydrate
post-embryonic development
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of lipid storage
positive regulation of cholesterol storage
lipid catabolic process
triglyceride catabolic process
cholesterol transport
sperm motility
cholesterol efflux
low-density lipoprotein particle remodeling
low-density lipoprotein particle clearance
lipoprotein metabolic process
lipoprotein biosynthetic process
lipoprotein catabolic process
cholesterol homeostasis
lipoprotein transport
regulation of cholesterol biosynthetic process
artery morphogenesis
response to virus
response to organic substance
response to selenium ion
response to lipopolysaccharide
cellular response to tumor necrosis factor
cellular response to prostaglandin stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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