Invitrogen

Apolipoprotein J Monoclonal Antibody (3R3/2)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-83879

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Flow Cytometry (Flow)

Assay-dependent
-

ELISA (ELISA)

Assay-dependent
-

Miscellaneous PubMed (MISC)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3R3/2

Immunogen

Glomerular basement membrane from a post-mortem kidney.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

BBS, pH 8.4

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_930015

Product Specific Information

A suggested positive control for immunohistochemical applications is kidney from post streptococcal glomerulonephritis patients.

Mouse anti Human clusterin antibody, clone 3R3/2 recognizes clusterin, an ubiquitously expressed protein of two 40 kDa chains, alpha and beta, covalently joined by disulfide bonds.

Target Information

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Aging associated protein 4; Aging-associated gene 4 protein; aging-associated protein 4; apo j; Apo J a; Apo J alpha; Apo J b; Apo J beta; Apo J α; Apo J β; Apo Ja; Apo Jb; Apo-J; ApoJ a; ApoJ alpha; ApoJ b; ApoJ beta; ApoJ α; ApoJ β; ApoJa; ApoJb; Apolipoprotein J; CLI; Clusterin; Clusterin alpha / beta chain; Complement associated protein SP40; Complement cytolysis inhibitor; complement lysis inhibitor; Complement-associated protein SP-40,40; GP80; HGNC:2095; Ku70-binding protein 1; Ku70-binding protein 1 (KUB1); MGC24903; NA1/NA2; SGP-2; Sulfated glycoprotein 2; Sulfated glycoprotein 2 (SGP2); Testosterone repressed prostate message 2 (TPRM2B); Testosterone-repressed prostate message 2; TRPM-2

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Gene Aliases: AAG4; APO-J; APOJ; CLI; CLU; CLU1; CLU2; KUB1; NA1/NA2; SGP-2; SGP2; SP-40; TRPM-2; TRPM2

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UniProt ID: (Human) P10909

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Entrez Gene ID: (Human) 1191

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Function(s)
protein binding ATPase activity ubiquitin protein ligase binding protein N-terminus binding chaperone binding misfolded protein binding
Process(es)
cell morphogenesis microglial cell activation release of cytochrome c from mitochondria platelet degranulation lipid metabolic process complement activation complement activation, classical pathway aging positive regulation of cell proliferation response to light stimulus response to wounding response to virus protein import endocrine pancreas development central nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process negative regulation of protein homooligomerization positive regulation of tumor necrosis factor production response to potassium ion reverse cholesterol transport estrous cycle innate immune response positive regulation of nitric oxide biosynthetic process positive regulation of cell differentiation neuron projection morphogenesis protein stabilization positive regulation of NF-kappaB transcription factor activity chaperone-mediated protein complex assembly response to misfolded protein chaperone-mediated protein folding microglial cell proliferation cellular response to growth factor stimulus regulation of beta-amyloid clearance regulation of neuron death positive regulation of neuron death positive regulation of beta-amyloid formation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of beta-amyloid formation regulation of neuronal signal transduction positive regulation of tau-protein kinase activity positive regulation of neurofibrillary tangle assembly positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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