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          • Primary Antibodies ›
          • Aquaporin 3 Antibodies

          Invitrogen

          Aquaporin 3 Polyclonal Antibody

          View all (16) Aquaporin 3 antibodies

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          Cite Aquaporin 3 Polyclonal Antibody

          • Antibody Testing Data (1)
          Aquaporin 3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Aquaporin 3 Antibody in Western Blot (WB)
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          FIGURE: 1 / 1

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          Aquaporin 3 Antibody (PA5-78810) in WB

          Western blot analysis of Aquaporin 3 in, Lane 1: mouse kidney tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat brain tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Aquaporin 3 Antibody in Western Blot (WB)

          Product Details

          PA5-78810

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to a sequence in the middle region of mouse Aquaporin 3 (122-137aa LYYDAIWAFANNELFV).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2745926

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: mouse kidney tissue, mouse brain tissue, rat kidney tissue, rat brain tissue.

          Target Information

          AQP3 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: 31.4 kDa water channel protein; AQP 3; AQP-3; Aquaglyceroporin-3; aquaporin 3 (GIL blood group); Aquaporin-3; Aquaporin3; Gill blood group

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          Gene Aliases: AQP-2; AQP-3; AQP3; GIL

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          UniProt ID: (Human) Q92482, (Rat) P47862, (Mouse) Q8R2N1

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          Entrez Gene ID: (Human) 360, (Rat) 65133, (Mouse) 11828

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          Function(s)
          transporter activity urea transmembrane transporter activity water channel activity glycerol channel activity
          Process(es)
          positive regulation of immune system process renal water homeostasis transport water transport excretion cellular water homeostasis glycerol transport response to retinoic acid response to vitamin D ion transmembrane transport odontogenesis regulation of keratinocyte differentiation response to calcium ion renal water absorption urea transmembrane transport urea transport carbohydrate transmembrane transport transmembrane transport
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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