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Invitrogen
Arginase 1 Polyclonal Antibody
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FIGURE: 1 / 6
Arginase 1 Antibody (PA5-18684) in ICC/IF
Immunocytochemistry analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Samples were incubated with Arginase 1 polyclonal antibody (Product # PA5-18684) using a dilution of 10 µg/mL for 1 hr followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa ... View More
Product Details
PA5-18684
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (NHKPETDYLKPPK) corresponding to the C-terminus amino acids of Arg1 (aa 311-323).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10980832
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A-I; AI type I arginase; Arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase
Gene Aliases: AI; AI256583; Arg-1; Arg1; PGIF
UniProt ID: (Rat) P07824, (Mouse) Q61176
Entrez Gene ID: (Rat) 29221, (Mouse) 11846
urea cycle
liver development
positive regulation of endothelial cell proliferation
arginine metabolic process
female pregnancy
aging
response to wounding
response to herbicide
response to manganese ion
response to zinc ion
response to selenium ion
regulation of L-arginine import
response to amine
arginine catabolic process to ornithine
lung development
response to lipopolysaccharide
collagen biosynthetic process
response to vitamin A
response to vitamin E
response to drug
response to amino acid
response to peptide hormone
response to cadmium ion
response to steroid hormone
response to axon injury
response to methylmercury
mammary gland involution
maternal process involved in female pregnancy
protein homotrimerization
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to glucagon stimulus
cellular response to dexamethasone stimulus
cellular response to transforming growth factor beta stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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