Invitrogen

Arginase 1 Polyclonal Antibody

Arginase 1 Antibody in Immunocytochemistry (ICC/IF)

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Arginase 1 Antibody (PA5-85267) in ICC/IF

Immunocytochemistry analysis of Arginase 1 in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Samples were incubated with Arginase 1 polyclonal antibody (Product # PA5-85267) using a dilution of 1:500 (green). Blue: Fluoroshield with DAPI. Scale bar = 10 μm.

Arginase 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Arginase 1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Arginase 1 Antibody in Western Blot (WB)

Arginase 1 Antibody in Flow Cytometry (Flow)

Arginase 1 Antibody in Immunoprecipitation (IP)

Arginase 1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA5-85267

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Flow Cytometry (Flow)

1:50-1:200

View 1 publication 1 publication

ELISA (ELISA)

1:1,000-1:10,000

Immunoprecipitation (IP)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human Arginase 1 Recombinant protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2792410

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (86%), Rat (86%), Pig (89%), Rabbit (91%), Bovine (90%).

Positive Control: Huh7, HepG2, mouse liver, rat liver.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CircZBTB44 promotes renal carcinoma progression by stabilizing HK3 mRNA structure.

Molecular cancer

Li T,Gu Y,Xu B,Kuca K,Zhang J,Wu W

Published figure using Arginase 1 polyclonal antibody (Product # PA5-85267) in Western Blot

Thu Apr 27 00:00:00 UTC 2023

A novel interleukin-2-based fusion molecule, HCW9302, differentially promotes regulatory T cell expansion to treat atherosclerosis in mice.

Frontiers in immunology

Zhu X,Li Q,George V,Spanoudis C,Gilkes C,Shrestha N,Liu B,Kong L,You L,Echeverri C,Li L,Wang Z,Chaturvedi P,Muniz GJ,Egan JO,Rhode PR,Wong HC

PA5-85267 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to find that HCW9302 exhibited a longer serum half-life with an approximately 1000-fold higher affinity for the IL-2Ra than IL-2.

Mon Feb 13 00:00:00 UTC 2023

Intestinal Flora Composition Determines Microglia Activation and Improves Epileptic Episode Progress.

Frontiers in cellular and infection microbiology

Ding X,Zhou J,Zhao L,Chen M,Wang S,Zhang M,Zhang X,Jiang G

Published figure using Arginase 1 polyclonal antibody (Product # PA5-85267) in Western Blot

Fri Apr 01 00:00:00 UTC 2022

Clusterin regulates macrophage expansion, polarization and phagocytic activity in response to inflammation in the kidneys.

Immunology and cell biology

Weng X,Zhao H,Guan Q,Shi G,Feng S,Gleave ME,Nguan CC,Du C

PA5-85267 was used in Immunohistochemistry to investigate the role of clusterin in macrophage polarization and phagocytosis.

Mon Mar 01 00:00:00 UTC 2021

Clusterin regulates macrophage expansion, polarization and phagocytic activity in response to inflammation in the kidneys.

Immunology and cell biology

Weng X,Zhao H,Guan Q,Shi G,Feng S,Gleave ME,Nguan CC,Du C

PA5-85267 was used in Immunohistochemistry to investigate the role of clusterin in macrophage polarization and phagocytosis.

Mon Mar 01 00:00:00 UTC 2021

DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization.

Cell death & disease

Liu L,Wu Y,Wang B,Jiang Y,Lin L,Li X,Yang S

Published figure using Arginase 1 polyclonal antibody (Product # PA5-85267) in Flow Cytometry

Mon May 17 00:00:00 UTC 2021

HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages.

Journal of nanobiotechnology

Niu Y,Stadler FJ,Yang X,Deng F,Liu G,Xia H

PA5-85267 was used in Western Blotting to investigate collagen nanofibres to stimulate urethral regeneration.

Wed Sep 22 00:00:00 UTC 2021

HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages.

Journal of nanobiotechnology

Niu Y,Stadler FJ,Yang X,Deng F,Liu G,Xia H

PA5-85267 was used in Western Blotting to investigate collagen nanofibres to stimulate urethral regeneration.

Wed Sep 22 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: A-I; AI type I arginase; Arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

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Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF

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UniProt ID: (Human) P05089, (Rat) P07824, (Mouse) Q61176

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Entrez Gene ID: (Human) 383, (Rat) 29221, (Mouse) 11846

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Function(s)

arginase activity manganese ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines metal ion binding

Process(es)

urea cycle liver development positive regulation of endothelial cell proliferation arginine metabolic process female pregnancy aging response to wounding response to herbicide response to manganese ion response to zinc ion response to selenium ion regulation of L-arginine import response to amine arginine catabolic process to ornithine lung development response to lipopolysaccharide collagen biosynthetic process response to vitamin A response to vitamin E response to drug response to amino acid response to peptide hormone response to cadmium ion response to steroid hormone response to axon injury response to methylmercury mammary gland involution maternal process involved in female pregnancy protein homotrimerization cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to interleukin-4 cellular response to glucagon stimulus cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus arginine catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Arginase 1 Polyclonal Antibody

Arginase 1 Antibody (PA5-85267) in ICC/IF

Product Details

PA5-85267

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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