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Invitrogen
Arginase 1 Recombinant Rabbit Monoclonal Antibody (24H4L3)
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FIGURE: 1 / 7
Arginase 1 Antibody (702730) in ICC/IF
For immunofluorescence analysis, Hep G2 cells were fixed and permeabilized for detection of endogenous Arginase 1 using Anti- Arginase 1 Recombinant Rabbit Monoclonal Antibody (Product # 702730, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for d... View More
Product Details
702730
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
24H4L3
Immunogen
Peptides corresponding to human ARG1 [1) aa304-aa322 2) aa52-aa71]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2734792
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Horse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A-I; AI type I arginase; Arginase; arginase 1 liver; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase
Gene Aliases: ARG1
UniProt ID: (Human) P05089, (Rat) P07824
Entrez Gene ID: (Human) 383, (Rat) 29221
urea cycle
liver development
positive regulation of endothelial cell proliferation
arginine metabolic process
female pregnancy
aging
response to wounding
response to herbicide
response to manganese ion
response to zinc ion
response to selenium ion
regulation of L-arginine import
response to amine
arginine catabolic process to ornithine
lung development
response to lipopolysaccharide
collagen biosynthetic process
response to vitamin A
response to vitamin E
response to drug
response to amino acid
response to peptide hormone
response to cadmium ion
response to steroid hormone
response to axon injury
response to methylmercury
mammary gland involution
maternal process involved in female pregnancy
protein homotrimerization
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to glucagon stimulus
cellular response to dexamethasone stimulus
cellular response to transforming growth factor beta stimulus
arginine catabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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