Invitrogen

Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor™ 700, eBioscience™

16 Published Figures

18 References

View all (93) Arginase 1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (16)

Arginase 1 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 17

Arginase 1 Antibody (56-3697-82) in Flow

C57BL/6 mouse bone marrow derived macrophages were polarized for 24 hours with either LPS (Product # 00-4976-93) and IFN-gamma (Product # 16-7311-85) to make M1 macrophages (left) or with IL-4 (Product # BMS338) to make M2a macrophages (right). Cells were surface stained with F4/80 Monoclonal Antibody, FITC (Product # 11-4801-82) then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.06 µg Arginase 1 Monoclonal Antibody, Alexa Fluor 700. Total viable cells we... View More

Arginase 1 Antibody in Immunocytochemistry (ICC/IF)

Arginase 1 Antibody in Immunohistochemistry (IHC)

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Product Details

56-3697-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A1exF5

Immunogen

E.coli-derived Recombinant mouse Arginase 1

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2734833

Product Specific Information

Description: The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1). This A1exF5 clone is compatible with both, the standard intracellular protocols, and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: This A1exF5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A1exF5 antibody has been tested by flow cytometric analysis of stimulated mouse bone marrow cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Timing of Interleukin-4 Stimulation of Macrophages Determines Their Anti-Microbial Activity during Infection with Salmonella enterica Serovar Typhimurium.

Cells

Brigo N,Neumaier E,Pfeifhofer-Obermair C,Grubwieser P,Engl S,Berger S,Seifert M,Reinstadler V,Oberacher H,Weiss G

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Fri Apr 14 00:00:00 UTC 2023

Repurposing nitric oxide donating drugs in cancer therapy through immune modulation.

Journal of experimental & clinical cancer research : CR

Li CY,Anuraga G,Chang CP,Weng TY,Hsu HP,Ta HDK,Su PF,Chiu PH,Yang SJ,Chen FW,Ye PH,Wang CY,Lai MD

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Sat Jan 14 00:00:00 UTC 2023

Discovery of a new marker to identify myeloid cells associated with metastatic breast tumours.

Cancer cell international

Bokil AA,Le Boulvais Børkja M,Wolowczyk C,Lamsal A,Prestvik WS,Nonstad U,Pettersen K,Andersen SB,Bofin AM,Bjørkøy G,Hak S,Giambelluca MS

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Sat Nov 18 00:00:00 UTC 2023

Advanced phosphocreatine-grafted chitosan hydrogel promote wound healing by macrophage modulation.

Frontiers in bioengineering and biotechnology

Sheng W,Qin H,Wang T,Zhao J,Fang C,Zhang P,Liu P,Udduttula A,Zeng H,Chen Y

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Wed May 31 00:00:00 UTC 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Sat Apr 01 00:00:00 UTC 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Tue Mar 14 00:00:00 UTC 2023

Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages.

Cellular & molecular biology letters

Gao T,Huang F,Wang W,Xie Y,Wang B

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Thu Mar 17 00:00:00 UTC 2022

Angiotensin 1-7 and its analogue decrease blood pressure but aggravate renal damage in preeclamptic mice.

Experimental animals

Liu Y,Zhai R,Tong J,Yu Y,Yang L,Gu Y,Niu J

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Thu Nov 10 00:00:00 UTC 2022

LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia.

Molecular medicine (Cambridge, Mass.)

Wu HY,Liu K,Zhang JL

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Sat Sep 24 00:00:00 UTC 2022

Gene-selective transcription promotes the inhibition of tissue reparative macrophages by TNF.

Life science alliance

Dichtl S,Sanin DE,Koss CK,Willenborg S,Petzold A,Tanzer MC,Dahl A,Kabat AM,Lindenthal L,Zeitler L,Satzinger S,Strasser A,Mann M,Roers A,Eming SA,El Kasmi KC,Pearce EJ,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Fri Apr 01 00:00:00 UTC 2022

Lactate and IL6 define separable paths of inflammatory metabolic adaptation.

Science advances

Dichtl S,Lindenthal L,Zeitler L,Behnke K,Schlösser D,Strobl B,Scheller J,El Kasmi KC,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Tue Jun 01 00:00:00 UTC 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Wed Oct 27 00:00:00 UTC 2021

Macrophage Metabolism of Apoptotic Cell-Derived Arginine Promotes Continual Efferocytosis and Resolution of Injury.

Cell metabolism

Yurdagul A,Subramanian M,Wang X,Crown SB,Ilkayeva OR,Darville L,Kolluru GK,Rymond CC,Gerlach BD,Zheng Z,Kuriakose G,Kevil CG,Koomen JM,Cleveland JL,Muoio DM,Tabas I

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Immunocytochemistry

Tue Mar 03 00:00:00 UTC 2020

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Immunohistochemistry

Fri Jul 17 00:00:00 UTC 2020

Chinese Medicine Formula PSORI-CM02 Alleviates Psoriatic Dermatitis via M-MDSCs and Th17 Crosstalk.

Frontiers in pharmacology

Deng J,Tan S,Liu R,Yu W,Chen H,Tang N,Han L,Lu C

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Sat Feb 06 00:00:00 UTC 2021

Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.

Cell death & disease

Hu Q,Peng J,Jiang L,Li W,Su Q,Zhang J,Li H,Song M,Cheng B,Xia J,Wu T

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Wed Oct 28 00:00:00 UTC 2020

Elevated granulocytic myeloid-derived suppressor cells are closely related with elevation of Th17 cells in mice with experimental asthma.

International journal of biological sciences

Xue F,Yu M,Li L,Zhang W,Ma Y,Dong L,Shan W,Zheng Y,Wang T,Feng D,Lv J,Wang X

56-3697 was used in Flow cytometry/Cell sorting to indicate that G-MDSC elevation plays a crucial role in asthmatic mice.

Fri Nov 12 00:00:00 UTC 2021

Regulation of the tumor immune microenvironment and vascular normalization in TNBC murine models by a novel peptide.

Oncoimmunology

Mirando AC,Patil A,Rafie CI,Christmas BJ,Pandey NB,Stearns V,Jaffee EM,Roussos Torres ET,Popel AS

Published figure using Arginase 1 monoclonal antibody (Product # 56-3697-82) in Flow Cytometry

Thu May 14 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: A-I; Arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

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Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF

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UniProt ID: (Human) P05089, (Mouse) Q61176

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Entrez Gene ID: (Human) 383, (Mouse) 11846

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Function(s)

arginase activity manganese ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines metal ion binding

Process(es)

urea cycle positive regulation of endothelial cell proliferation arginine metabolic process regulation of L-arginine import arginine catabolic process to ornithine collagen biosynthetic process protein homotrimerization liver development arginine catabolic process aging response to herbicide response to manganese ion response to zinc ion response to selenium ion response to amine lung development response to vitamin A response to vitamin E response to drug response to amino acid response to cadmium ion response to axon injury response to methylmercury mammary gland involution maternal process involved in female pregnancy cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to interleukin-4 cellular response to glucagon stimulus cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor™ 700, eBioscience™

Arginase 1 Antibody (56-3697-82) in Flow

Product Details

56-3697-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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