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Invitrogen
Arp2 Polyclonal Antibody
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Arp2 Antibody (PA5-143649) in WB
Western blot of Arp2 in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 polyclonal antibody (Product # PA5-143649) and Phospho-Arp2 (Thr237, Thr238) polyclonal antibody (Product # PA5-143650), then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.
Product Details
PA5-143649
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Arp2 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Arp2. This peptide sequence is highly conserved in rat, mouse, chicken, and frog Arp2 proteins.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942877
Product Specific Information
The antibody detects 44 and 32 kDa* proteins corresponding to the molecular mass of Arp2 on SDS-PAGE immunoblots of human A431, HUVEC, HeLa, and Jurkat cells as well as rat PC12, mouse C2C12, and rabbit fibroblasts.
Target Information
ARP2 (actin-related protein 2) is an ATP-binding component of the ARP2/3 complex - a multiprotein complex that mediates actin polymerization upon stimulation by the nucleation-promoting factor (NPF). The ARP2/3 complex mediates the formation of branched actin networks in the cytoplasm and provides the force for cell motility. The ARP2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Actin-like protein 2; Actin-related protein 2; ARP2 actin-related protein 2 homolog
Gene Aliases: 4921510D23Rik; AA409782; ACTR2; ARP2; D6Ertd746e
UniProt ID: (Human) P61160, (Rat) Q5M7U6, (Mouse) P61161
Entrez Gene ID: (Human) 10097, (Rat) 289820, (Mouse) 66713
movement of cell or subcellular component
establishment or maintenance of cell polarity
asymmetric cell division
meiotic chromosome movement towards spindle pole
cytosolic transport
meiotic cytokinesis
Arp2/3 complex-mediated actin nucleation
Fc-gamma receptor signaling pathway involved in phagocytosis
cilium assembly
ephrin receptor signaling pathway
spindle localization
actin cytoskeleton organization
meiotic cell cycle
actin filament organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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