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Invitrogen
Arylsulfatase A Monoclonal Antibody (4C10)
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Arylsulfatase A Antibody (MA5-33005) in IHC (P)
Immunohistochemical analysis of ARSA in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-ARSA antibody (Product # MA5-33005) overnight at 4°C. Biotinylated goat anti-rabbit IgG wa... View More
Product Details
MA5-33005
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
4C10
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA (454-482aa QALKQLQLLKAQLDAAVTFGPSQVARGED).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802639
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat testis tissue, rat liver tissue, rat brain tissue, rat lung tissue, mouse testis tissue, mouse liver tissue, mouse brain tissue, mouse lung tissue, mouse HEPA1-6 whole cell, human A375 whole cell, human A549 whole cell, human SMMC-7721 whole cell. IHC: human lung cancer tissue. Flow: ANA-1 cell, Raji cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy, a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARSA-I; ARSA1; Arylsulfatase A; ASA; ASNA-I; ATPase ASNA1; Cerebroside-sulfatase; EC 3.1.6.8; GET3; hASNA-I; TRC40
Gene Aliases: ARSA; As-2; AS-A; As2; ASA; AW212749; MLD; TISP73
UniProt ID: (Human) P15289, (Mouse) P50428
Entrez Gene ID: (Human) 410, (Rat) 315222, (Mouse) 11883
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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