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Invitrogen
Asialo GM1 Polyclonal Antibody, Functional Grade, eBioscience™
FIGURE: 1 / 1
Asialo GM1 Antibody (16-6507-39) in Flow
Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 PerCP-Cy5-5 (Product # 45-5941-82) (left) or Anti-Mouse CD11b APC (Product # 17-0112-82) (right) and Anti-Asialo-GM1 Functional Grade Purified.An Anti-Rabbit PE (Product # 12-4739-81) was used for detection.Total viable cells were used for analysis.
Product Details
16-6507-39
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Conjugate
Form
Lyophilized
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10718540
Product Specific Information
Description: This rabbit polyclonal antibody reacts with the asialo ganglio-N-tetraosylceramide (asialo-GM1), a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression. The antibody recognizes asialo-GM1 specifically and not other glycolipids, including GM1 and asialo-GM2.
This antibody has been reported to deplete NK cells in mice and hamsters in vivo and exhibits in vitro effects in a variety of species.
This polyclonal antibody has been reported for use in flow cytometric analysis, depletion of NK cells, and in vitro functional studies. Intravenous and IP injection of 20-50 µL has been shown to decrease NK1.1 populations after 3-4 days. Repeat injections may be necessary.
Applications Tested: This polyclonal antibody against asialo-GM1 has been tested by flow cytometric analysis of mouse splenocytes. Optimal staining concentration will vary depending on the lot. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Endotoxin: Less than 0.001 ng/µg antibody as determined by the LAL assay.
Storage and handling: It is recommended to add 1 mL of sterile (endotoxin free) room temperature water. After reconstitution pass through 0.22 µm filter prior to injection.
Target Information
Asialo ganglio-N-tetraosylceramide (asialo-GM1) is a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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