Invitrogen

Asialo GM1 Polyclonal Antibody, Alexa Fluor™ 488, eBioscience™

Asialo GM1 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Asialo GM1 Antibody (53-6507-80) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.5 µg of Anti-Asialo-GM1 Alexa Fluor® 488. Total viable cells were used for analysis.

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Product Details

53-6507-80

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Rat

Published species

Hamster

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10717257

Product Specific Information

Description: This rabbit polyclonal antibody reacts with the asialo ganglio-N-tetraosylceramide (asialo-GM1), a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression. The antibody recognizes asialo-GM1 specifically and not other glycolipids, including GM1 and asialo-GM2.

This antibody has been reported to deplete NK cells in mice and hamsters in vivo and exhibits in vitro effects in a variety of species.

Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis.

Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. Because this is a polyclonal antibody, there may be differences in staining brightness from lot to lot. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Asialo ganglio-N-tetraosylceramide (asialo-GM1) is a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Development of a Syrian hamster anti-PD-L1 monoclonal antibody enables oncolytic adenoviral immunotherapy modelling in an immunocompetent virus replication permissive setting.

Frontiers in immunology

Clubb JHA,Kudling TV,Girych M,Haybout L,Pakola S,Hamdan F,Cervera-Carrascon V,Hemmes A,Grönberg-Vähä-Koskela S,Santos JM,Quixabeira DCA,Basnet S,Heiniö C,Arias V,Jirovec E,Kaptan S,Havunen R,Sorsa S,Erikat A,Schwartz J,Anttila M,Aro K,Viitala T,Vattulainen I,Cerullo V,Kanerva A,Hemminki A

53-6507-80 was used in Flow cytometry/Cell sorting to develop an anti-Syrian hamster PD-L1 mAb to enable the evaluation of safety and efficacy, when combining anti-PD-L1 with an oncolytic adenovirus encoding tumour necrosis factor alpha (TNFα) and interleukin-2 (IL-2) (Ad5/3-E2F-D24-hTNFα-IRES-hIL-2 or TILT-123).

Mon Feb 27 00:00:00 UTC 2023

Oncolytic Adenovirus Coding for a Variant Interleukin 2 (vIL-2) Cytokine Re-Programs the Tumor Microenvironment and Confers Enhanced Tumor Control.

Frontiers in immunology

Quixabeira DCA,Zafar S,Santos JM,Cervera-Carrascon V,Havunen R,Kudling TV,Basnet S,Anttila M,Kanerva A,Hemminki A

53-6507-80 was used in Flow Cytometry to conclude that Ad5/3-E2F-d24-vIL2 is a promising candidate for translation into clinical trials in human immunosuppressive solid tumors.

Fri Oct 15 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: AsialoGM1

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