Proteintech

Ataxin 2 Polyclonal Antibody

Ataxin 2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

Ataxin 2 Antibody (21776-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 21776-1-AP (Ataxin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Ataxin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Ataxin 2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

21776-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 12 publications 12 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Ataxin 2 Fusion Protein Ag16470 (251-600 aa encoded by BC114546)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GSDQRVVNG GVPWPSPCPS PSSRPPSRYQ SGPNSLPPRA ATPTRPPSRP PSRPSRPPSH PSAHGSPAPV STMPKRMSSE GPPRMSPKAQ RHPRNHRVSA GRGSISSGLE FVSHNPPSEA ATPPVARTSP SGGTWSSVVS GVPRLSPKTH RPRSPRQNSI GNTPSGPVLA SPQAGIIPTE AVAMPIPAAS PTPASPASNR AVTPSSEAKD SRLQDQRQNS PAGNKENIKP NETSPSFSKA ENKGISPVVS EHRKQIDDLK KFKNDFRLQP SSTSESMDQL LNKNREGEKS RDLIKDKIEP SAKDSFIENS SSNCTSGSSK PNSPSISPSI LSNTEHKRGP EVTSQGVQTS S (251-600 aa encoded by BC114546)

Target Information

The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. Defects in this gene are the cause of spinocerebellar ataxia type 2 (SCA2). SCA2 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA2 is caused by expansion of a CAG repeat in the coding region of this gene. Longer expansions result in earlier onset of the disease. Alternatively spliced transcript variants encoding different isoforms have been identified but their full length sequence has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules.

Cell

Cui Q,Bi H,Lv Z,Wu Q,Hua J,Gu B,Huo C,Tang M,Chen Y,Chen C,Chen S,Zhang X,Wu Z,Lao Z,Sheng N,Shen C,Zhang Y,Wu ZY,Jin Z,Yang P,Liu H,Li J,Bai G

Thu Feb 16 00:00:00 UTC 2023

TR-FRET-Based Immunoassay to Measure Ataxin-2 as a Target Engagement Marker in Spinocerebellar Ataxia Type 2.

Molecular neurobiology

Bux J,Sen NE,Klink IM,Hauser S,Synofzik M,Schöls L,Auburger G,Riess O,Hübener-Schmid J

Thu Jun 01 00:00:00 UTC 2023

Suboptimal Mitochondrial Activity Facilitates Nuclear Heat Shock Responses for Proteostasis and Genome Stability.

Molecules and cells

Park D,Yu Y,Kim JH,Lee J,Park J,Hong K,Seo JK,Lim C,Min KT

Fri Jun 30 00:00:00 UTC 2023

NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility.

Nature communications

Gleixner AM,Verdone BM,Otte CG,Anderson EN,Ramesh N,Shapiro OR,Gale JR,Mauna JC,Mann JR,Copley KE,Daley EL,Ortega JA,Cicardi ME,Kiskinis E,Kofler J,Pandey UB,Trotti D,Donnelly CJ

Mon Jun 13 00:00:00 UTC 2022

Genome-wide CRISPR screen reveals v-ATPase as a drug target to lower levels of ALS protein ataxin-2.

Cell reports

Kim G,Nakayama L,Blum JA,Akiyama T,Boeynaems S,Chakraborty M,Couthouis J,Tassoni-Tsuchida E,Rodriguez CM,Bassik MC,Gitler AD

Tue Oct 25 00:00:00 UTC 2022

Genome-wide CRISPR screen reveals v-ATPase as a drug target to lower levels of ALS protein ataxin-2.

Cell reports

Kim G,Nakayama L,Blum JA,Akiyama T,Boeynaems S,Chakraborty M,Couthouis J,Tassoni-Tsuchida E,Rodriguez CM,Bassik MC,Gitler AD

Tue Oct 25 00:00:00 UTC 2022

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Diabetologia

Zhang M,Yang C,Zhu M,Qian L,Luo Y,Cheng H,Geng R,Xu X,Qian C,Liu Y

Sat May 01 00:00:00 UTC 2021

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Diabetologia

Zhang M,Yang C,Zhu M,Qian L,Luo Y,Cheng H,Geng R,Xu X,Qian C,Liu Y

Sat May 01 00:00:00 UTC 2021

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Diabetologia

Zhang M,Yang C,Zhu M,Qian L,Luo Y,Cheng H,Geng R,Xu X,Qian C,Liu Y

Sat May 01 00:00:00 UTC 2021

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Diabetologia

Zhang M,Yang C,Zhu M,Qian L,Luo Y,Cheng H,Geng R,Xu X,Qian C,Liu Y

Sat May 01 00:00:00 UTC 2021

Atxn2-CAG100-KnockIn mouse spinal cord shows progressive TDP43 pathology associated with cholesterol biosynthesis suppression.

Neurobiology of disease

Canet-Pons J,Sen NE,Arsović A,Almaguer-Mederos LE,Halbach MV,Key J,Döring C,Kerksiek A,Picchiarelli G,Cassel R,René F,Dieterlé S,Fuchs NV,König R,Dupuis L,Lütjohann D,Gispert S,Auburger G

Sat May 01 00:00:00 UTC 2021

Autism-Misregulated eIF4G Microexons Control Synaptic Translation and Higher Order Cognitive Functions.

Molecular cell

Gonatopoulos-Pournatzis T,Niibori R,Salter EW,Weatheritt RJ,Tsang B,Farhangmehr S,Liang X,Braunschweig U,Roth J,Zhang S,Henderson T,Sharma E,Quesnel-Vallières M,Permanyer J,Maier S,Georgiou J,Irimia M,Sonenberg N,Forman-Kay JD,Gingras AC,Collingridge GL,Woodin MA,Cordes SP,Blencowe BJ

Thu Mar 19 00:00:00 UTC 2020

Mid-Gestation lethality of Atxn2l-Ablated Mice.

International journal of molecular sciences

Key J,Harter PN,Sen NE,Gradhand E,Auburger G,Gispert S

Mon Jul 20 00:00:00 UTC 2020

Hexanucleotide Repeat Expansions in c9FTD/ALS and SCA36 Confer Selective Patterns of Neurodegeneration In Vivo.

Cell reports

Todd TW,McEachin ZT,Chew J,Burch AR,Jansen-West K,Tong J,Yue M,Song Y,Castanedes-Casey M,Kurti A,Dunmore JH,Fryer JD,Zhang YJ,San Millan B,Teijeira Bautista S,Arias M,Dickson D,Gendron TF,Sobrido MJ,Disney MD,Bassell GJ,Rossoll W,Petrucelli L

Tue May 05 00:00:00 UTC 2020

LSM12-EPAC1 defines a neuroprotective pathway that sustains the nucleocytoplasmic RAN gradient.

PLoS biology

Lee J,Park J,Kim JH,Lee G,Park TE,Yoon KJ,Kim YK,Lim C

Tue Dec 01 00:00:00 UTC 2020

LSM12-EPAC1 defines a neuroprotective pathway that sustains the nucleocytoplasmic RAN gradient.

PLoS biology

Lee J,Park J,Kim JH,Lee G,Park TE,Yoon KJ,Kim YK,Lim C

Tue Dec 01 00:00:00 UTC 2020

Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD.

Neuron

Fang MY,Markmiller S,Vu AQ,Javaherian A,Dowdle WE,Jolivet P,Bushway PJ,Castello NA,Baral A,Chan MY,Linsley JW,Linsley D,Mercola M,Finkbeiner S,Lecuyer E,Lewcock JW,Yeo GW

Wed Sep 04 00:00:00 UTC 2019

Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD.

Neuron

Fang MY,Markmiller S,Vu AQ,Javaherian A,Dowdle WE,Jolivet P,Bushway PJ,Castello NA,Baral A,Chan MY,Linsley JW,Linsley D,Mercola M,Finkbeiner S,Lecuyer E,Lewcock JW,Yeo GW

Wed Sep 04 00:00:00 UTC 2019

RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43.

Neuron

Mann JR,Gleixner AM,Mauna JC,Gomes E,DeChellis-Marks MR,Needham PG,Copley KE,Hurtle B,Portz B,Pyles NJ,Guo L,Calder CB,Wills ZP,Pandey UB,Kofler JK,Brodsky JL,Thathiah A,Shorter J,Donnelly CJ

Wed Apr 17 00:00:00 UTC 2019

Internalization Characterization of Si Nanorod with Camouflaged Cell Membrane Proteins Reveals ATXN2 as a Negative Regulator.

Cells

Lu Y,Dai J,Kong N,Liu J,Gong J,Yao Y

Mon Aug 19 00:00:00 UTC 2019

Calcium-responsive transactivator (CREST) toxicity is rescued by loss of PBP1/ATXN2 function in a novel yeast proteinopathy model and in transgenic flies.

PLoS genetics

Park S,Park SK,Watanabe N,Hashimoto T,Iwatsubo T,Shelkovnikova TA,Liebman SW

Thu Aug 01 00:00:00 UTC 2019

Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.

Cell reports

Shelkovnikova TA,An H,Skelt L,Tregoning JS,Humphreys IR,Buchman VL

Tue Dec 24 00:00:00 UTC 2019

Aberrant deposition of stress granule-resident proteins linked to C9orf72-associated TDP-43 proteinopathy.

Molecular neurodegeneration

Chew J,Cook C,Gendron TF,Jansen-West K,Del Rosso G,Daughrity LM,Castanedes-Casey M,Kurti A,Stankowski JN,Disney MD,Rothstein JD,Dickson DW,Fryer JD,Zhang YJ,Petrucelli L

Fri Feb 15 00:00:00 UTC 2019

Aberrant deposition of stress granule-resident proteins linked to C9orf72-associated TDP-43 proteinopathy.

Molecular neurodegeneration

Chew J,Cook C,Gendron TF,Jansen-West K,Del Rosso G,Daughrity LM,Castanedes-Casey M,Kurti A,Stankowski JN,Disney MD,Rothstein JD,Dickson DW,Fryer JD,Zhang YJ,Petrucelli L

Fri Feb 15 00:00:00 UTC 2019

Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly(PR) toxicity.

Science (New York, N.Y.)

Zhang YJ,Guo L,Gonzales PK,Gendron TF,Wu Y,Jansen-West K,O'Raw AD,Pickles SR,Prudencio M,Carlomagno Y,Gachechiladze MA,Ludwig C,Tian R,Chew J,DeTure M,Lin WL,Tong J,Daughrity LM,Yue M,Song Y,Andersen JW,Castanedes-Casey M,Kurti A,Datta A,Antognetti G,McCampbell A,Rademakers R,Oskarsson B,Dickson DW,Kampmann M,Ward ME,Fryer JD,Link CD,Shorter J,Petrucelli L

Fri Feb 15 00:00:00 UTC 2019

Generation of an Atxn2-CAG100 knock-in mouse reveals N-acetylaspartate production deficit due to early Nat8l dysregulation.

Neurobiology of disease

Sen NE,Canet-Pons J,Halbach MV,Arsovic A,Pilatus U,Chae WH,Kaya ZE,Seidel K,Rollmann E,Mittelbronn M,Meierhofer D,De Zeeuw CI,Bosman LWJ,Gispert S,Auburger G

Sun Dec 01 00:00:00 UTC 2019

Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology.

eLife

Zhang P,Fan B,Yang P,Temirov J,Messing J,Kim HJ,Taylor JP

Wed Mar 20 00:00:00 UTC 2019

Zika Virus Subverts Stress Granules To Promote and Restrict Viral Gene Expression.

Journal of virology

Bonenfant G,Williams N,Netzband R,Schwarz MC,Evans MJ,Pager CT

Sat Jun 15 00:00:00 UTC 2019

Zika Virus Subverts Stress Granules To Promote and Restrict Viral Gene Expression.

Journal of virology

Bonenfant G,Williams N,Netzband R,Schwarz MC,Evans MJ,Pager CT

Sat Jun 15 00:00:00 UTC 2019

Conserved Pbp1/Ataxin-2 regulates retrotransposon activity and connects polyglutamine expansion-driven protein aggregation to lifespan-controlling rDNA repeats.

Communications biology

Ostrowski LA,Hall AC,Szafranski KJ,Oshidari R,Abraham KJ,Chan JNY,Krustev C,Zhang K,Wang A,Liu Y,Guo R,Mekhail K

Sat Jan 11 00:00:00 UTC 2020

Conserved Pbp1/Ataxin-2 regulates retrotransposon activity and connects polyglutamine expansion-driven protein aggregation to lifespan-controlling rDNA repeats.

Communications biology

Ostrowski LA,Hall AC,Szafranski KJ,Oshidari R,Abraham KJ,Chan JNY,Krustev C,Zhang K,Wang A,Liu Y,Guo R,Mekhail K

Sat Jan 11 00:00:00 UTC 2020

Atxn2 Knockout and CAG42-Knock-in Cerebellum Shows Similarly Dysregulated Expression in Calcium Homeostasis Pathway.

Cerebellum (London, England)

Halbach MV,Gispert S,Stehning T,Damrath E,Walter M,Auburger G

Wed Feb 01 00:00:00 UTC 2017

Atxn2 Knockout and CAG42-Knock-in Cerebellum Shows Similarly Dysregulated Expression in Calcium Homeostasis Pathway.

Cerebellum (London, England)

Halbach MV,Gispert S,Stehning T,Damrath E,Walter M,Auburger G

Wed Feb 01 00:00:00 UTC 2017

TDP-43 and FUS RNA-binding proteins bind distinct sets of cytoplasmic messenger RNAs and differently regulate their post-transcriptional fate in motoneuron-like cells.

The Journal of biological chemistry

Colombrita C,Onesto E,Megiorni F,Pizzuti A,Baralle FE,Buratti E,Silani V,Ratti A

Fri May 04 00:00:00 UTC 2012

TDP-43 and FUS RNA-binding proteins bind distinct sets of cytoplasmic messenger RNAs and differently regulate their post-transcriptional fate in motoneuron-like cells.

The Journal of biological chemistry

Colombrita C,Onesto E,Megiorni F,Pizzuti A,Baralle FE,Buratti E,Silani V,Ratti A

Fri May 04 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Ataxin-2; FLJ46772; spinocerebellar ataxia 2 (olivopontocerebellar ataxia 2, autosomal dominant, ataxin 2); spinocerebellar ataxia 2 homolog; Spinocerebellar ataxia type 2 protein; Spinocerebellar ataxia type 2 protein homolog; Trinucleotide repeat-containing gene 13 protein

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Gene Aliases: 9630045M23Rik; ASL13; ATX2; ATXN2; AW544490; SCA2; TNRC13

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UniProt ID: (Human) Q99700, (Mouse) O70305

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Entrez Gene ID: (Human) 6311, (Rat) 288663, (Mouse) 20239

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Function(s)

RNA binding epidermal growth factor receptor binding protein binding protein C-terminus binding poly(A) RNA binding molecular_function DBD domain binding

Process(es)

negative regulation of receptor internalization biological_process cerebellar Purkinje cell differentiation cytoplasmic mRNA processing body assembly stress granule assembly negative regulation of multicellular organism growth neuron projection morphogenesis homeostasis of number of cells neuromuscular process regulation of translation regulation of cytoplasmic mRNA processing body assembly RNA metabolic process RNA transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ataxin 2 Polyclonal Antibody

Ataxin 2 Antibody (21776-1-AP) in ICC/IF

Product Details

21776-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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