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Invitrogen
Ataxin 3 Monoclonal Antibody (2SCA-1H9)
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FIGURE: 1 / 4
Ataxin 3 Antibody (MA3-082) in ICC/IF
Immunofluorescent analysis of Ataxin 3 (green) in Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Ataxin 3 mouse monoclonal antibody (Product # MA3-082) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28... View More
Product Details
MA3-082
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2SCA-1H9
Immunogen
Human Ataxin-3 fragment from aa F112-aa L249
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633327
Product Specific Information
MA3-082 detects Ataxin 3 from human, mouse and rat samples.
MA3-082 has been successfully used in Western blot, immunofluorescence, and ELISA applications.
MA3-082 has been successfully used in Western blot, immunofluorescence, and ELISA applications. The MA3-082 immunogen is the human ataxin-3 fragment from aa 112 - aa L249 as a fusion protein.
Target Information
Machado-Joseph disease is an autosomal dominant neurologic disorder, and is now known to be the same as previously described spinocerebellar ataxia-3. MJD protein (Ataxin-3) contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. This protein interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription, and also acts as a histone-binding protein that regulates transcription. MJD is a deubiquitinating enzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ataxin 3 variant an; ataxin 3 variant ao; ataxin 3 variant at; ataxin 3 variant e; ataxin 3 variant h; ataxin 3 variant m; ataxin 3 variant r; ataxin 3 variant ref; ataxin 3 variant y; Ataxin-3; ataxin3; josephin; Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3); Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) homolog; Machado-Joseph disease protein 1; Machado-Joseph disease protein 1 homolog; olivopontocerebellar ataxia 3; spin; Spinocerebellar ataxia type 3 protein
Gene Aliases: 2210008M02Rik; AI463012; AI647473; AT3; ataxin-3; ATX3; ATXN3; JOS; MJD; MJD1; Rsca3; SCA3
UniProt ID: (Human) P54252, (Rat) O35815, (Mouse) Q9CVD2
Entrez Gene ID: (Human) 4287, (Rat) 60331, (Mouse) 110616
RNA polymerase II regulatory region DNA binding
histone deacetylase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
omega peptidase activity
ubiquitin protein ligase binding
identical protein binding
ATPase binding
Lys63-specific deubiquitinase activity
Lys48-specific deubiquitinase activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
microtubule cytoskeleton organization
transcription, DNA-templated
regulation of transcription, DNA-templated
ubiquitin-dependent protein catabolic process
misfolded or incompletely synthesized protein catabolic process
regulation of cell-substrate adhesion
actin cytoskeleton organization
cellular response to heat
monoubiquitinated protein deubiquitination
exploration behavior
proteasome-mediated ubiquitin-dependent protein catabolic process
intermediate filament cytoskeleton organization
protein K63-linked deubiquitination
histone H3 deacetylation
protein K48-linked deubiquitination
cellular response to misfolded protein
proteolysis
nucleotide-excision repair
chemical synaptic transmission
nervous system development
protein deubiquitination
positive regulation of ERAD pathway
protein localization to cytosolic proteasome complex involved in ERAD pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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