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Ataxin 7 Antibody (MA3-083) in ICC/IF
Immunofluorescent analysis of Ataxin 7 (green) in Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Ataxin 7 mouse monoclonal antibody (Product # MA3-083) at a dilution of 1:50 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Alexa Fluor 488 conjugate (Product # A281... View More
Product Details
MA3-083
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
3SCA-1C1
Immunogen
Recombinant human Ataxin 7 aa 1 - 229.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633328
Product Specific Information
MA3-083 detects Ataxin 7 from human and mouse samples.
MA3-083 has been successfully used in Western blot, immunofluorescence, and ELISA applications.
The MA3-083 epitope is mapped between aa 66 and aa 119.
Target Information
Spinocerebellar ataxia type 7 (SCA7) belongs to a class of hereditary neurodegenrative diseases characterized by abnormally expanded polyglutamine (CAG) repeats at the N-terminus. This autosomal dominant cerebellar ataxia primarily affects the cerebellum, retina, and brain stem and causes dementia, macular degeneration and other neurodegenerative characteristics. SCA7 gene encodes the ubiquitous 892 residue protein ataxin-7. This protein is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ataxin-7; spinocerebellar ataxia 7 homolog; Spinocerebellar ataxia type 7 protein; Spinocerebellar ataxia type 7 protein homolog
Gene Aliases: A430107N12Rik; ADCAII; AI627028; ataxin-7; ATXN7; OPCA3; SCA7
UniProt ID: (Human) O15265, (Mouse) Q8R4I1
Entrez Gene ID: (Human) 6314, (Mouse) 246103
microtubule cytoskeleton organization
transcription, DNA-templated
regulation of transcription, DNA-templated
histone deubiquitination
negative regulation of phosphorylation
negative regulation of insulin-like growth factor receptor signaling pathway
positive regulation of transcription from RNA polymerase II promoter
nucleus organization
visual perception
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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