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Invitrogen
Aurora A/B/C Monoclonal Antibody (4A7)
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FIGURE: 1 / 2
Aurora A/B/C Antibody (MA5-47425) in ICC/IF
Immunocytochemistry analysis of Aurora A/B/C in HeLa cell cultures. Samples were stained with Aurora A/B/C monoclonal antibody (Product # MA5-47425) in green. Strong staining in spindle poles is seen in cells at anaphase and the antibody also stains the midbodies between daughter cells.Cells were counterstained with chicken polyclonal antibody to vimentinin red, revealing cytoplasmic intermediate filaments.... View More
Product Details
MA5-47425
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Horse,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4A7
Immunogen
Full length recombinant human AURKA protein expressed from E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 50% glycerol
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942407
Target Information
Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIM-1; ARK-1; ARK-2; ARK-3; Aurora 1; Aurora 2; Aurora 3; aurora A; aurora B; aurora family kinase 1; Aurora kinase A; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora kinase C; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; Aurora-A; aurora-B; aurora-related kinase 1; aurora-related kinase 2; aurora-related kinase 3; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; Aurora/IPL1-related kinase 2; Aurora/IPL1-related kinase 3; Aurora/IPL1/Eg2 protein 1; Aurora/IPL1/Eg2 protein 2; Breast tumor-amplified kinase; epididymis secretory protein Li 90; Ipl1- and aurora-related kinase 1; protein phosphatase 1, regulatory subunit 47; protein phosphatase 1, regulatory subunit 48; ratAurA; serine/threonine kinase 12; serine/threonine kinase 13 (aurora/IPL-like); serine/threonine kinase 13 (aurora/IPL1-like); serine/threonine kinase 5; serine/threonine kinase 6; serine/threonine protein kinase 15; serine/threonine protein kinase 6; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase aurora-B; Serine/threonine-protein kinase aurora-C; Serine/threonine-protein kinase Ayk1; STK-1
Gene Aliases: AIE1; AIE2; AIK; AIK2; AIK3; AIM-1; AIM1; AIRK1; AIRK2; AIRK3; AL022959; ARK-1; ARK-2; ARK-3; ARK1; ARK2; ARK3; AU019385; AURA; AurB; AurC; AURKA; AURKB; aurkb-sv1; aurkb-sv2; AURKC; Aurora-A; aurora-C; AW539821; AYK1; BOS_13320; BOS_18197; BOS_18918; BTAK; CR956640.2; HEL-S-90; IAK; IAK1; IAK3; IPL1; PPP1R47; PPP1R48; SPGF5; STK-1; STK1; STK12; STK13; STK15; STK5; STK6; STK7
protein kinase activity
protein serine/threonine kinase activity
ATP binding
protein kinase binding
ubiquitin protein ligase binding
protein serine/threonine/tyrosine kinase activity
protein binding
histone serine kinase activity
metal ion binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
negative regulation of transcription from RNA polymerase II promoter
negative regulation of B cell apoptotic process
positive regulation of cytokinesis
protein localization to kinetochore
cellular response to UV
cleavage furrow formation
mitotic spindle midzone assembly
meiotic spindle organization
microtubule cytoskeleton organization
mitotic cell cycle
protein phosphorylation
spindle organization
mitotic spindle organization
spindle assembly involved in female meiosis I
mitotic nuclear division
mitotic centrosome separation
meiotic nuclear division
anterior/posterior axis specification
cell projection organization
regulation of protein stability
negative regulation of protein binding
response to estradiol
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of cytokinesis
histone-serine phosphorylation
negative regulation of apoptotic process
centrosome organization
cell division
centrosome localization
protein localization to centrosome
positive regulation of oocyte maturation
neuron projection extension
cell cycle
regulation of signal transduction
phosphorylation
meiotic cell cycle
aging
cell proliferation
abscission
negative regulation of cytokinesis
histone H3-S28 phosphorylation
sister chromatid cohesion
attachment of spindle microtubules to kinetochore
histone modification
protein sumoylation
anaphase-promoting complex-dependent catabolic process
spindle checkpoint
positive regulation of telomere maintenance via telomerase
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
protein autophosphorylation
positive regulation of telomerase activity
regulation of chromosome segregation
regulation of signal transduction by p53 class mediator
positive regulation of telomere capping
negative regulation of spindle checkpoint
G2/M transition of mitotic cell cycle
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
positive regulation of mitotic nuclear division
regulation of centrosome cycle
cytokinesis
spermatogenesis
oocyte development
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