Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Aurora B Recombinant Rabbit Monoclonal Antibody (RM278)
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 6
Aurora B Antibody (MA5-27890) in ICC/IF
Immunofluorescence analysis of Aurora B was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Aurora B Recombinant Rabbit Monoclonal Antibody (RM278) (Product # MA5-27890) at 1:200 dilution in 0.1% BSA and incubated for overnight at 4°C and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alex... View More
Product Details
MA5-27890
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM278
Immunogen
A peptide corresponding to the N-terminus of human Aurora kinase B
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2744944
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: AIM-1; ARK-2; Aurora 1; aurora B; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; aurora-B; aurora-related kinase 2; Aurora/IPL1-related kinase 2; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine kinase 5; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; STK-1
Gene Aliases: AIK2; AIM-1; AIM1; AIRK2; AL022959; ARK2; AurB; AURKB; aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK-1; STK1; STK12; STK5
UniProt ID: (Human) Q96GD4, (Mouse) O70126
Entrez Gene ID: (Human) 9212, (Mouse) 20877
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
histone serine kinase activity
metal ion binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
negative regulation of transcription from RNA polymerase II promoter
negative regulation of B cell apoptotic process
protein phosphorylation
spindle organization
sister chromatid cohesion
aging
cell proliferation
attachment of spindle microtubules to kinetochore
abscission
histone modification
protein sumoylation
anaphase-promoting complex-dependent catabolic process
spindle checkpoint
negative regulation of protein binding
positive regulation of telomere maintenance via telomerase
negative regulation of cytokinesis
positive regulation of cytokinesis
protein localization to kinetochore
cellular response to UV
cleavage furrow formation
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
histone H3-S28 phosphorylation
protein autophosphorylation
mitotic spindle midzone assembly
positive regulation of telomerase activity
regulation of chromosome segregation
regulation of signal transduction by p53 class mediator
positive regulation of telomere capping
cell cycle
mitotic spindle organization
mitotic nuclear division
phosphorylation
regulation of cytokinesis
cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support