Invitrogen

Aurora C Polyclonal Antibody

Aurora C Antibody in Immunocytochemistry (ICC/IF)

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Aurora C Antibody (38-9400) in ICC/IF

Immunofluorescence analysis of Aurora C was performed using 70% confluent log phase HeLa cells treated with 3 uM of Nocodazole for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Aurora C Rabbit Polyclonal Antibody (Product # 38-9400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary... View More

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Product Details

38-9400

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-3 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of the human Aurora C (Serine/threonine-protein kinase 13, Serine/threonine kinase AIE2, Aurora/IPL1/Eg2 protein 2, Aurora/IPL1-related kinase 3) protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533392

Target Information

AURKC (Aurora C) is a chromosomal passenger protein that forms complexes with AURKB (Aurora B) and inner centromere proteins, playing a role in organizing microtubules in relation to centrosome/spindle function during mitosis. It is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex.

PloS one

Sasai K,Katayama H,Hawke DH,Sen S

38-9400 was used in immunoprecipitation and western blot to characterize shared and distinct features compared with aurora-B chromosome passenger protein complex by Aurora-C interactions with INCENP and survivin

Wed Jul 26 00:00:00 UTC 2017

Overexpression of active Aurora-C kinase results in cell transformation and tumour formation.

PloS one

Khan J,Ezan F,Crémet JY,Fautrel A,Gilot D,Lambert M,Benaud C,Troadec MB,Prigent C

38-9400 was used in western blot to study the contribution of Aurora-A , -B, and -C to cancer.

Mon Mar 26 00:00:00 UTC 2012

Overexpression of active Aurora-C kinase results in cell transformation and tumour formation.

PloS one

Khan J,Ezan F,Crémet JY,Fautrel A,Gilot D,Lambert M,Benaud C,Troadec MB,Prigent C

38-9400 was used in western blot to study the contribution of Aurora-A , -B, and -C to cancer.

Mon Mar 26 00:00:00 UTC 2012

Overexpression of active Aurora-C kinase results in cell transformation and tumour formation.

PloS one

Khan J,Ezan F,Crémet JY,Fautrel A,Gilot D,Lambert M,Benaud C,Troadec MB,Prigent C

38-9400 was used in western blot to study the contribution of Aurora-A , -B, and -C to cancer.

Mon Mar 26 00:00:00 UTC 2012

Overexpression of active Aurora-C kinase results in cell transformation and tumour formation.

PloS one

Khan J,Ezan F,Crémet JY,Fautrel A,Gilot D,Lambert M,Benaud C,Troadec MB,Prigent C

38-9400 was used in western blot to study the contribution of Aurora-A , -B, and -C to cancer.

Mon Mar 26 00:00:00 UTC 2012

Anaplastic thyroid carcinoma: expression profile of targets for therapy offers new insights for disease treatment.

Annals of surgical oncology

Wiseman SM,Masoudi H,Niblock P,Turbin D,Rajput A,Hay J,Bugis S,Filipenko D,Huntsman D,Gilks B

Thu Feb 01 00:00:00 UTC 2007

Aurora-B regulates RNA methyltransferase NSUN2.

Molecular biology of the cell

Sakita-Suto S,Kanda A,Suzuki F,Sato S,Takata T,Tatsuka M

Thu Mar 01 00:00:00 UTC 2007

Aurora-B regulates RNA methyltransferase NSUN2.

Molecular biology of the cell

Sakita-Suto S,Kanda A,Suzuki F,Sato S,Takata T,Tatsuka M

Thu Mar 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: ARK-3; Aurora 3; aurora B; Aurora kinase C; aurora-related kinase 3; Aurora/IPL1-related kinase 3; Aurora/IPL1/Eg2 protein 1; Aurora/IPL1/Eg2 protein 2; epididymis secretory protein Li 90; serine/threonine kinase 13 (aurora/IPL-like); serine/threonine kinase 13 (aurora/IPL1-like); Serine/threonine-protein kinase 13; Serine/threonine-protein kinase aurora-C

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Gene Aliases: AIE1; AIE2; AIK3; AIRK3; ARK-3; ARK3; AurC; AURKC; aurora-C; HEL-S-90; IAK3; SPGF5; STK13

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UniProt ID: (Human) Q9UQB9, (Mouse) O88445

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Entrez Gene ID: (Human) 6795, (Mouse) 20871

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Function(s)

protein kinase activity protein serine/threonine/tyrosine kinase activity ATP binding histone serine kinase activity nucleotide binding protein serine/threonine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

protein phosphorylation cell cycle mitotic spindle organization mitotic nuclear division regulation of signal transduction phosphorylation regulation of cytokinesis histone-serine phosphorylation cell division meiotic cell cycle cytokinesis spermatogenesis attachment of spindle microtubules to kinetochore histone modification positive regulation of cytokinesis oocyte development mitotic spindle midzone assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Aurora C Polyclonal Antibody

Aurora C Antibody (38-9400) in ICC/IF

Product Details

38-9400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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