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Invitrogen
Auxin response factor Monoclonal Antibody (14D2)
FIGURE: 1 / 2
Auxin response factor Antibody (MA5-34937) in IHC (P)
Immunohistochemistry analysis of Auxin response factor in paraffin-embedded leafbud. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Auxin response factor monoclonal antibody (Product # MA5-34937) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (m... View More
Product Details
MA5-34937
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
14D2
Immunogen
Recombinant protein within tea Auxin response factor aa 401-586
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848843
Product Specific Information
Positive Control: Tea leafbud.
Target Information
Auxin response factors (ARFs) are transcription factors that bind to auxin response elements in promoters of early auxin response genes and play central roles in many auxin-mediated processes. As transcription factors, ARFs possess a modular structure and consist of three major domains: an amino-terminal DNA-binding domain (DBD); a middle domain, which is responsible for gene activation or repression; and a carboxy-terminal PB1(Phox and Bem1) domain, contained within a region that was previously called domain III/I. ARFs function as transcription activators or repressors, depending on the middle region of the ARF protein; Q-rich middle regions activate transcription, whereas P/S/T-rich middle regions confer repressor activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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