Invitrogen

Axl Monoclonal Antibody (C.995.0)

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Axl Antibody (MA5-14855) in WB

CRISPR-Cas9 mediated genome editing ofAxl (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR932417_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Fig (a) Western blot analysis of AXL was performed by loading 30 µg of U-87 MG Cas9 (Lane 1), and U-87 MG Cas9 cells transduced with Axl Lentiviral sgRNA (Lane 2) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins... View More

Axl Antibody in Immunocytochemistry (ICC/IF)

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Axl Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across U-87 MG, U-2 OS, T98G,SH-SY5Y, HEK-293 owing to their inherent genetic constitution. Relative expression of Axl was observed as high in U-87 MG, U-2 OS and T98G as compared to SH-SY5Y and HEK-293 using Anti-Axl Monoclonal Antibody (C.995.0) (Product # MA5-14855) in Western Blot. {RE}

Product Details

MA5-14855

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

5 µg

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

C.995.0

Immunogen

Recombinant human fragment of the Axl protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

64 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10986568

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with related proteins.

Target Information

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AXL oncogene; AXL transforming sequence/gene; AZF1; oncogene AXL; sAXL; soluble AXL; Tyrosine-protein kinase receptor UFO

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Gene Aliases: ARK; AXL; JTK11; Tyro7; UFO

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UniProt ID: (Human) P30530

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Entrez Gene ID: (Human) 558

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Function(s)

virus receptor activity phosphatidylserine binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity protein binding ATP binding protein heterodimerization activity

Process(es)

neuron migration natural killer cell differentiation positive regulation of cytokine-mediated signaling pathway blood vessel remodeling phagocytosis inflammatory response signal transduction spermatogenesis peptidyl-tyrosine phosphorylation forebrain cell migration platelet activation cellular response to extracellular stimulus negative regulation of interferon-gamma production negative regulation of tumor necrosis factor production positive regulation of natural killer cell differentiation secretion by cell erythrocyte homeostasis substrate adhesion-dependent cell spreading cellular response to interferon-alpha ovulation cycle apoptotic cell clearance protein kinase B signaling negative regulation of neuron apoptotic process innate immune response viral entry into host cell vascular endothelial growth factor receptor signaling pathway cell maturation positive regulation of pinocytosis negative regulation of lymphocyte activation positive regulation of protein kinase B signaling vagina development cellular response to lipopolysaccharide dendritic cell differentiation negative regulation of dendritic cell apoptotic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Axl Monoclonal Antibody (C.995.0)

Axl Antibody (MA5-14855) in WB

Product Details

MA5-14855

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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