Invitrogen

B-Raf Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-95203

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human B Raf recombinant protein (Position: A38-V230).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807007

Product Specific Information

Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human SH-SY5Y whole cell, rat brain tissue, rat testis tissue, mouse brain tissue. IHC: human liver cancer tissue, human lung cancer tissue, human placenta tissue, human differentiated adenocarcinoma of the rectum tissue, mouse brain tissue, rat brain tissue. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer, and is thought to play a role in the postsynaptic responses of hippocampal neuron. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in B-Raf have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for B-Raf.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); FLJ95109; MGC126806; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1

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Gene Aliases: 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; B-RAF1; BRAF; Braf-2; BRAF1; Braf2; C230098H17; C87398; D6Ertd631e; NS7; RAFB1

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UniProt ID: (Human) P15056, (Mouse) P28028

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Entrez Gene ID: (Human) 673, (Rat) 114486, (Mouse) 109880

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity calcium ion binding protein binding ATP binding small GTPase binding mitogen-activated protein kinase kinase binding identical protein binding protein heterodimerization activity nucleotide binding receptor signaling protein activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding
Process(es)
MAPK cascade activation of MAPKK activity myeloid progenitor cell differentiation protein phosphorylation visual learning positive regulation of gene expression negative regulation of fibroblast migration cell differentiation positive regulation of peptidyl-serine phosphorylation somatic stem cell population maintenance cellular response to drug regulation of cell proliferation negative regulation of apoptotic process CD4-positive, alpha-beta T cell differentiation positive T cell selection response to peptide hormone negative regulation of neuron apoptotic process alpha-beta T cell differentiation regulation of axon regeneration positive regulation of axon regeneration positive regulation of axonogenesis protein heterooligomerization positive regulation of stress fiber assembly response to cAMP long-term synaptic potentiation head morphogenesis positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion positive regulation of substrate adhesion-dependent cell spreading negative regulation of synaptic vesicle exocytosis negative regulation of endothelial cell apoptotic process signal transduction glucose transport phosphorylation intracellular signal transduction establishment of protein localization to membrane organ morphogenesis thyroid gland development thymus development face development
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