Invitrogen

B-Raf (K601E Mutant) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-143383

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide surrounding mutant K601E of human B-Raf. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.54 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2938378

Product Specific Information

Store as concentrated solution.

Centrifuge briefly prior to opening vial.

Target Information

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases|This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion|Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung|Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes|A pseudogene of this gene has been identified on the X chromosome

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-raf; B-Raf proto-oncogene, serine/threonine kinase; B-RAF1; BRAF1; casein kinase 1, alpha 1; casein kinase 1, alpha 1-like; casein kinase I alpha S-like; Casein kinase I isoform alpha; Casein kinase I isoform alpha-like; CASP-1; CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase-1; CK1; CKI-alpha; CKI-alpha-like; clock regulator kinase; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; NS7; p45; RAFB1

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Gene Aliases: CASP1; CK1; CK1a; CKIa; CSNK1A1; CSNK1A1L; HEL-S-77p; HLCDGP1; ICE; IL1BC; IL1BCE; P45; PRO2975

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UniProt ID: (Human) P29466, (Human) P48729, (Human) Q8N752

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Entrez Gene ID: (Human) 834, (Human) 1452, (Human) 122011

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Function(s)
endopeptidase activity cysteine-type endopeptidase activity protein binding cysteine-type endopeptidase activator activity involved in apoptotic process cysteine-type endopeptidase activity involved in apoptotic process protein kinase activity protein serine/threonine kinase activity ATP binding kinase activity
Process(es)
response to hypoxia proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process signal transduction regulation of autophagy protein processing response to lipopolysaccharide interleukin-1 beta production response to ATP regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion regulation of inflammatory response mitochondrial depolarization membrane hyperpolarization pyroptosis cellular response to mechanical stimulus cellular response to organic substance programmed necrotic cell death toxin transport cell morphogenesis phagocytosis regulation of cell shape Wnt signaling pathway peptidyl-serine phosphorylation protein phosphorylation Golgi organization mitotic nuclear division cell surface receptor signaling pathway positive regulation of proteasomal ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process intermediate filament cytoskeleton organization cell division negative regulation of canonical Wnt signaling pathway regulation of GTP binding beta-catenin destruction complex assembly beta-catenin destruction complex disassembly
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