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B3GNT1 Antibody (PA5-47860) in WB
Western blot analysis of B3GNT1 in human muscle tissue and mouse muscle tissue. Samples were incubated in B3GNT1 polyclonal antibody (Product # PA5-47860) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for β-1,4-Glucuronyltransferase 1/B4GAT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47860
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CHO-derived recombinant human beta-1,3-N-acetylglucosaminyltransferase 1/B3GNT1 Asp43-Cys415
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610270
Product Specific Information
In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) B4GalT1 and rhB3GNT 2 is observed.
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
Beta-1,4-glucuronyltransferase involved in O- mannosylation of alpha-dystroglycan (DAG1). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE, during synthesis of phosphorylated O- mannosyl glycan (PubMed:25279699, PubMed:25279697). Phosphorylated O-mannosyl glycan is a carbohydrate is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:25279699, PubMed:25279697). Required for axon guidance; via its function in O-mannosylation of alpha- dystroglycan (DAG1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B3GNT1 (HGNC:15685); beta-1,3-N-acetylglucosaminyltransferase bGnT-6; Beta-1,4-glucuronyltransferase 1; EC 2.4.1.149; I-beta-1,3-N-acetylglucosaminyltransferase; iGnT; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
Gene Aliases: 1500032M01Rik; B3GN-T1; B3GNT1; B3GNT6; B4GAT1; BETA3GNT1; BETA3GNTI; iGAT; iGNT; MDDGA13
UniProt ID: (Human) O43505, (Mouse) Q8BWP8
Entrez Gene ID: (Human) 11041, (Mouse) 108902
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity
glucuronosyltransferase activity
metal ion binding
alpha-1,6-mannosyltransferase activity
alpha-1,2-mannosyltransferase activity
mannosyltransferase activity
alpha-1,3-mannosyltransferase activity
alpha-1,3-galactosyltransferase activity
UDP-glucose:glycoprotein glucosyltransferase activity
glycolipid mannosyltransferase activity
oligosaccharyl transferase activity
dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity
acetylglucosaminyltransferase activity
acetylgalactosaminyltransferase activity
galactosyltransferase activity
fucosyltransferase activity
O antigen polymerase activity
lipopolysaccharide-1,6-galactosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
cellulose synthase activity
9-phenanthrol UDP-glucuronosyltransferase activity
1-phenanthrol glycosyltransferase activity
9-phenanthrol glycosyltransferase activity
1,2-dihydroxy-phenanthrene glycosyltransferase activity
phenanthrol glycosyltransferase activity
beta-1,4-mannosyltransferase activity
alpha-1,2-galactosyltransferase activity
dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity
lipopolysaccharide-1,5-galactosyltransferase activity
dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity
dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity
inositol phosphoceramide synthase activity
glucosyltransferase activity
alpha-(1->3)-fucosyltransferase activity
alpha-(1->6)-fucosyltransferase activity
indole-3-butyrate beta-glucosyltransferase activity
salicylic acid glucosyltransferase (ester-forming) activity
salicylic acid glucosyltransferase (glucoside-forming) activity
benzoic acid glucosyltransferase activity
chondroitin hydrolase activity
dolichyl-pyrophosphate Man7GlcNAc2 alpha-1,6-mannosyltransferase activity
cytokinin 9-beta-glucosyltransferase activity
Performance Guarantee
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