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BAG2 Antibody (MA5-49282) in WB
Western blot analysis of BAG2 using BAG2 antibody (Product # MA5-49282). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates; Lane 2: human Hela whole cell lysates; Lane 3: human HepG2 whole cell lysates; Lane 4: human K562 whole cell lysates; Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membran... View More
Product Details
MA5-49282
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8F11G2
Immunogen
E.coli-derived human BAG2 recombinant protein (Position: M1-N211).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074940
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human BAG2 shares 93.4% amino acid (aa) sequence identity with mouse BAG2.
Positive Control - WB: human Jurkat whole cell, human Hela whole cell, human HepG2 whole cell, human K562 whole cell, mouse liver tissue. Flow: K562 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAG family molecular chaperone regulator 2; BAG-2; BAG-family molecular chaperone regulator-2; Bcl-2-associated athanogene 2; BCL2-associated athanogene 2; dJ417I1.2 (BAG-family molecular chaperone regulator 2); KIAA0576; MGC149462; RP3-496N17.2
Gene Aliases: 2610042A13Rik; BAG-2; BAG2; BC016230; dJ417I1.2
UniProt ID: (Human) O95816, (Mouse) Q91YN9
Entrez Gene ID: (Human) 9532, (Mouse) 213539
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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