Invitrogen

BAK1 Monoclonal Antibody (4C2)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-36225

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

ELISA (ELISA)

-
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

4C2

Immunogen

E.coli-derived human BAK/BAK1 recombinant protein (Position: A22-S211).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2884059

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human 293T whole cell, human A431 whole cell, human THP-1 whole cell, human MCF-7 whole cell, human PC-3 whole cell. IHC: human intestinal cancer tissue, human mammary cancer tissue, human tonsil tissue, human lung cancer tissue, mouse intestine tissue, rat intestine tissue. ICC/IF: A431 cell. Flow: PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Apoptosis regulator BAK; Bcl-2 homologous antagonist/killer; Bcl-2-like protein 7; Bcl2 homologous antagonist/killer; BCL2-antagonist/killer 1; Bcl2-L-7; BCL2-like 7 protein; BCL2-like 7protein; MGC117255; MGC3887; pro-apoptotic protein BAK

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Gene Aliases: BAK; BAK-LIKE; BAK1; BCL2L7; CDN1; N-Bak; N-BAK1

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UniProt ID: (Human) Q16611, (Mouse) O08734

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Entrez Gene ID: (Human) 578, (Mouse) 12018, (Rat) 116502

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Function(s)
protein binding heat shock protein binding identical protein binding protein homodimerization activity ion channel binding metal ion binding protein heterodimerization activity chaperone binding BH domain binding
Process(es)
leukocyte homeostasis B cell homeostasis B cell apoptotic process release of cytochrome c from mitochondria blood vessel remodeling myeloid cell homeostasis B cell negative selection apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process mitochondrial fusion cell proliferation negative regulation of cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to fungus response to mycotoxin establishment or maintenance of transmembrane electrochemical gradient response to gamma radiation positive regulation of calcium ion transport into cytosol negative regulation of gene expression cytolysis negative regulation of endoplasmic reticulum calcium ion concentration negative regulation of peptidyl-serine phosphorylation cellular response to UV limb morphogenesis regulation of apoptotic process positive regulation of apoptotic process regulation of protein homodimerization activity regulation of protein heterodimerization activity fibroblast apoptotic process positive regulation of proteolysis regulation of mitochondrial membrane permeability post-embryonic camera-type eye morphogenesis homeostasis of number of cells regulation of cell cycle regulation of mitochondrial membrane potential vagina development intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress thymocyte apoptotic process positive regulation of release of cytochrome c from mitochondria apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand activation of cysteine-type endopeptidase activity positive regulation of endoplasmic reticulum unfolded protein response apoptotic process involved in patterning of blood vessels brain development aging response to UV-C response to organic cyclic compound endocrine pancreas development organ regeneration response to drug response to hydrogen peroxide response to ethanol cellular response to mechanical stimulus activation of signaling protein activity involved in unfolded protein response endoplasmic reticulum calcium ion homeostasis positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of IRE1-mediated unfolded protein response
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