Invitrogen

BAP31 Monoclonal Antibody (CC-1)

BAP31 Antibody in Immunocytochemistry (ICC/IF)

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BAP31 Antibody (MA3-002) in ICC/IF

Immunofluorescence analysis of BAP31 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with BAP31 Monoclonal Antibody (Product # MA3-002) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11006) at a dilution of 1:200... View More

BAP31 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

BAP31 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with BAP31 siRNA and reduction of signal was observed in Western Blot using BAP31 Monoclonal Antibody (Product # MA3-002). {KD}

Product Details

MA3-002

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,500-1:6,000

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:1,000-1:10,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 8 publications 8 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Non-human primate

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

CC-1

Immunogen

Amino acid residues 230-246 of BAP31 protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325095

Product Specific Information

MA3-002 detects BAP31 from human, non-human primate, bovine, mouse and hamster samples.

MA3-002 has been successfully used in Western blot, ELISA, immunoprecipitation, immunofluorescent, and immunohistochemical procedures. By Western blot, this antibody detects a 28-kDa protein corresponding to human BAP31.

The MA3-002 antigen is solubilized protein from human neuroendocrine cell line IMR-32 corresponding to the C-terminal region residues 230-246 of BAP31 protein. This sequence is conserved in non-human primate, bovine and hamster species.

Target Information

BAP31 (B-Cell Receptor Associated Protein, 31-kDa) is a multiple membrane spanning protein of the endoplasmic reticulum. It has been shown to form both homo- and hetero-oligomers with the closely related BAP29, and is a potential apoptotic regulator as a predicted BCL-2/BCL-XL-associated protein. In the C-terminal domain of BAP31, there are two identical recognition sites for initiator Caspases 1 and 8 of the programmed death cascade. It is hypothesized that BAP31 plays a role in the transport of selected proteins to the Golgi apparatus. BAP31 has also been shown to control expression of CFTR (cystic fibrosis transmembrane conductance regulator). It is believed that interference with the expression or function of BAP31 in epithelial cells may be a way to circumvent the chloride channel defect in cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Components of the LINC and NPC complexes coordinately target and translocate a virus into the nucleus to promote infection.

PLoS pathogens

Spriggs CC,Cha G,Li J,Tsai B

Published figure using BAP31 monoclonal antibody (Product # MA3-002) in Immunocytochemistry

Thu Sep 01 00:00:00 UTC 2022

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

Cell host & microbe

Walch P,Selkrig J,Knodler LA,Rettel M,Stein F,Fernandez K,Viéitez C,Potel CM,Scholzen K,Geyer M,Rottner K,Steele-Mortimer O,Savitski MM,Holden DW,Typas A

MA3-002 was used in Immunocytochemistry to provide a method for probing host-pathogen PPIs during infection and a resource for interrogating STm effector mechanisms.

Wed Aug 11 00:00:00 UTC 2021

Lunapark-dependent formation of a virus-induced ER exit site contains multi-tubular ER junctions that promote viral ER-to-cytosol escape.

Cell reports

Bagchi P,Liu X,Cho WJ,Tsai B

MA3-002 was used in Flow cytometry/Cell sorting to suggest that ER tubular junctions are vulnerable sites exploited by viruses for membrane penetration.

Tue Dec 07 00:00:00 UTC 2021

Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes.

The Journal of cell biology

Chen YJ,Williams JM,Arvan P,Tsai B

MA3-002 was used in Immunocytochemistry to demonstrate that the cytosolic leaflet ER morphogenic protein reticulon (RTN) protects ER membrane integrity when polyomavirus SV40 escapes the ER to reach the cytosol en route to infection.

Mon Feb 03 00:00:00 UTC 2020

Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes.

The Journal of cell biology

Chen YJ,Williams JM,Arvan P,Tsai B

Mon Feb 03 00:00:00 UTC 2020

Golgi-associated BICD adaptors couple ER membrane penetration and disassembly of a viral cargo.

The Journal of cell biology

Spriggs CC,Badieyan S,Verhey KJ,Cianfrocco MA,Tsai B

Published figure using BAP31 monoclonal antibody (Product # MA3-002) in Immunocytochemistry

Mon May 04 00:00:00 UTC 2020

TBC1D5-Catalyzed Cycling of Rab7 Is Required for Retromer-Mediated Human Papillomavirus Trafficking during Virus Entry.

Cell reports

Xie J,Heim EN,Crite M,DiMaio D

MA3-002 was used in Immunocytochemistry to select artificial proteins that modulate cellular proteins required for HPV infection and discover that entry requires TBC1D5, a retromer-associated, Rab7-specific GTPase-activating protein.

Tue Jun 09 00:00:00 UTC 2020

Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.

eLife

Miller-Vedam LE,Bräuning B,Popova KD,Schirle Oakdale NT,Bonnar JL,Prabu JR,Boydston EA,Sevillano N,Shurtleff MJ,Stroud RM,Craik CS,Schulman BA,Frost A,Weissman JS

MA3-002 was used in Western Blot to illuminate the structural and mechanistic basis of endoplasmic reticulum membrane protein complex multifunctionality and invstigate its role in differentially regulating the biogenesis of distinct client protein classes.

Wed Nov 25 00:00:00 UTC 2020

Dynein Engages and Disassembles Cytosol-Localized Simian Virus 40 To Promote Infection.

Journal of virology

Ravindran MS,Spriggs CC,Verhey KJ,Tsai B

MA3-002 was used in Immunocytochemistry-immunoflourescence to study the mechanism by which PyV is transported from the cytosol into the nucleus during infection.

Fri Jun 15 00:00:00 UTC 2018

A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane.

PLoS pathogens

Ravindran MS,Bagchi P,Inoue T,Tsai B

MA3-002 was used in immunocytochemistry to discover that Hsp105 cooperates with Hsc70 to facilitate virus entry

Sat Aug 01 00:00:00 UTC 2015

A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane.

PLoS pathogens

Ravindran MS,Bagchi P,Inoue T,Tsai B

MA3-002 was used in immunocytochemistry to discover that Hsp105 cooperates with Hsc70 to facilitate virus entry

Sat Aug 01 00:00:00 UTC 2015

MUC1 regulates nuclear localization and function of the epidermal growth factor receptor.

Journal of cell science

Bitler BG,Goverdhan A,Schroeder JA

MA3-002 was used in western blot to investigate the regulation of EGFR by MUC1

Sat May 15 00:00:00 UTC 2010

BAP31 is involved in the retention of cytochrome P450 2C2 in the endoplasmic reticulum.

The Journal of biological chemistry

Szczesna-Skorupa E,Kemper B

MA3-002 was used in immunoprecipitation to demonstrate the interaction between BAP31 and P450 2C2 in the ER of transfected cells and its effect on the subcellular localization and accumulation of P450.

Fri Feb 17 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: 6C6 antigen; 6C6-AG tumor-associated antigen; accessory protein BAP31; B-cell receptor-associated protein 31; BCR-associated protein 31; BCR-associated protein Bap31; CDM protein; OTTHUMP00000025978; p28; p28 Bap31; Protein CDM

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Gene Aliases: 6C6-AG; BAP31; BCAP31; BOS_25461; CDM; DDCH; DXS1357E

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UniProt ID: (Human) P51572, (Mouse) Q61335

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Entrez Gene ID: (Human) 10134, (Bovine) 533949, (Chinese hamster) 100770274, (Mouse) 27061

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Function(s)

protein binding protein complex binding MHC class I protein binding

Process(es)

antigen processing and presentation of peptide antigen via MHC class I intracellular protein transport ER to Golgi vesicle-mediated transport cellular component disassembly involved in execution phase of apoptosis positive regulation of cytosolic calcium ion concentration spermatogenesis negative regulation of endoplasmic reticulum calcium ion concentration calcium-mediated signaling using intracellular calcium source positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of mitochondrial calcium ion concentration protein localization to endoplasmic reticulum exit site positive regulation of ER-associated ubiquitin-dependent protein catabolic process positive regulation of retrograde protein transport, ER to cytosol positive regulation of intrinsic apoptotic signaling pathway transport apoptotic process immune response protein transport vesicle-mediated transport

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BAP31 Monoclonal Antibody (CC-1)

BAP31 Antibody (MA3-002) in ICC/IF

Product Details

MA3-002

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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