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BBS10 Antibody (PA5-97161) in WB
Western blot analysis of BBS10 in extracts of various cell lines. Samples were incubated with BBS10 Polyclonal antibody (Product # PA5-97161) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-97161
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 474-723 of human BBS10 (NP_0789613)
Conjugate
Form
Liquid
Concentration
1.78 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808963
Product Specific Information
Immunogen sequence: AENKDALEKT QTYLKVHSNL VIPDVELETY IPYSTPTLTP TDTFQTVETL TCLSLERNRL TDYYEPLLKN NSTAYSTRGN RIEISYENLQ VTNITRKGSM LPVSCKLPNM GTSQSYLSSS MPAGCVLPVG GNFEILLHYY LLNYAKKCHQ SEETMVSMII ANALLGIPKV LYKSKTGKYS FPHTYIRAVH ALQTNQPLVS SQTGLESVMG KYQLLTSVLQ CLTKILTIDM VITVKRHPQK VHNQDSEDEL; Positive Samples: A-549, A-431, HT-1080, HeLa, HepG2, Mouse liver; Cellular Location: Cell projection, cilium
Target Information
BBS10 is a member of the Bardet-Biedl syndrome (BBS) gene family. Bardet-Biedl syndrome is an autosomal recessive disorder characterized by progressive retinal degeneration, obesity, polydactyly, renal malformation and mental retardation. The proteins encoded by BBS gene family members are structurally diverse and the similar phenotypes exhibited by mutations in BBS gene family members is likely due to their shared roles in cilia formation and function. Many BBS proteins localize to the basal bodies, ciliary axonemes, and pericentriolar regions of cells. BBS proteins may also be involved in intracellular trafficking via microtubule-related transport. The protein encoded by this gene is likely not a ciliary protein but rather has distant sequence homology to type II chaperonins. As a molecular chaperone, this protein may affect the folding or stability of other ciliary or basal body proteins. Inhibition of this protein's expression impairs ciliogenesis in preadipocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bardet-Biedl syndrome 10 protein; Bardet-Biedl syndrome 10 protein homolog; BBSome complex assembly protein BBS10
Gene Aliases: 1300007O09Rik; AI452285; BBS10; C12orf58
UniProt ID: (Human) Q8TAM1, (Mouse) Q9DBI2
Entrez Gene ID: (Human) 79738, (Mouse) 71769
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