Proteintech

BBS3 Polyclonal Antibody

BBS3 Antibody in Immunocytochemistry (ICC/IF)

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BBS3 Antibody (12676-1-AP) in ICC/IF

Immunofluorescent analysis of MDCK cells using 12676-1-AP (BBS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

BBS3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BBS3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12676-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BBS3 Fusion Protein Ag3363 (1-186 aa encoded by BC024239)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGLLDRLSV LLGLKKKEVH VLCLGLDNSG KTTIINKLKP SNAQSQNILP TIGFSIEKFK SSSLSFTVFD MSGQGRYRNL WEHYYKEGQA IIFVIDSSDR LRMVVAKEEL DTLLNHPDIK HRRIPILFFA NKMDLRDAVT SVKVSQLLCL ENIKDKPWHI CASDAIKGEG LQEGVDWLQD QIQTVKT (1-186 aa encoded by BC024239)

Target Information

The protein encoded by this gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP-binding proteins which are involved in regulation of intracellular traffic. Mutations in this gene are associated with Bardet-Biedl syndrome (BBS). Alternative splicing results in multiple transcript variants of this gene. A vision-specific transcript encoding a different protein has been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.

Biology open

Fujisawa S,Qiu H,Nozaki S,Chiba S,Katoh Y,Nakayama K

Wed Sep 15 00:00:00 UTC 2021

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

The EMBO journal

Duan S,Li H,Zhang Y,Yang S,Chen Y,Qiu B,Huang C,Wang J,Li J,Zhu X,Yan X

Fri Jan 15 00:00:00 UTC 2021

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

The EMBO journal

Duan S,Li H,Zhang Y,Yang S,Chen Y,Qiu B,Huang C,Wang J,Li J,Zhu X,Yan X

Fri Jan 15 00:00:00 UTC 2021

Absence of BBSome function leads to astrocyte reactivity in the brain.

Molecular brain

Singh M,Garrison JE,Wang K,Sheffield VC

Thu May 09 00:00:00 UTC 2019

Requirement of IFT-B-BBSome complex interaction in export of GPR161 from cilia.

Biology open

Nozaki S,Castro Araya RF,Katoh Y,Nakayama K

Tue Sep 17 00:00:00 UTC 2019

Bardet-Biedl syndrome 3 regulates the development of cranial base midline structures.

Bone

Kawasaki M,Izu Y,Hayata T,Ideno H,Nifuji A,Sheffield VC,Ezura Y,Noda M

Tue Aug 01 00:00:00 UTC 2017

Bardet-Biedl syndrome 3 regulates the development of cranial base midline structures.

Bone

Kawasaki M,Izu Y,Hayata T,Ideno H,Nifuji A,Sheffield VC,Ezura Y,Noda M

Tue Aug 01 00:00:00 UTC 2017

Bardet-Biedl syndrome 3 regulates the development of cranial base midline structures.

Bone

Kawasaki M,Izu Y,Hayata T,Ideno H,Nifuji A,Sheffield VC,Ezura Y,Noda M

Tue Aug 01 00:00:00 UTC 2017

BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.

PLoS genetics

Hsu Y,Garrison JE,Kim G,Schmitz AR,Searby CC,Zhang Q,Datta P,Nishimura DY,Seo S,Sheffield VC

Sun Oct 01 00:00:00 UTC 2017

BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.

PLoS genetics

Hsu Y,Garrison JE,Kim G,Schmitz AR,Searby CC,Zhang Q,Datta P,Nishimura DY,Seo S,Sheffield VC

Sun Oct 01 00:00:00 UTC 2017

Basal body proteins regulate Notch signaling through endosomal trafficking.

Journal of cell science

Leitch CC,Lodh S,Prieto-Echagüe V,Badano JL,Zaghloul NA

Sun Jun 01 00:00:00 UTC 2014

Basal body proteins regulate Notch signaling through endosomal trafficking.

Journal of cell science

Leitch CC,Lodh S,Prieto-Echagüe V,Badano JL,Zaghloul NA

Sun Jun 01 00:00:00 UTC 2014

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Proceedings of the National Academy of Sciences of the United States of America

Humbert MC,Weihbrecht K,Searby CC,Li Y,Pope RM,Sheffield VC,Seo S

Tue Nov 27 00:00:00 UTC 2012

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Proceedings of the National Academy of Sciences of the United States of America

Humbert MC,Weihbrecht K,Searby CC,Li Y,Pope RM,Sheffield VC,Seo S

Tue Nov 27 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: ADP-ribosylation factor-like protein 6; ADP-ribosylation-like 6; ADP-ribosylation-like factor homolog ARL6; Bardet-Biedl syndrome 3 protein

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Gene Aliases: 1110018H24Rik; 2210411E14Rik; ARL6; BBS3; RP55

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UniProt ID: (Human) Q9H0F7, (Mouse) O88848

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Entrez Gene ID: (Human) 84100, (Rat) 363760, (Mouse) 56297

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Function(s)

protein binding GTP binding phospholipid binding metal ion binding molecular_function nucleotide binding

Process(es)

protein targeting to membrane small GTPase mediated signal transduction determination of left/right symmetry brain development visual perception regulation of smoothened signaling pathway retina layer formation Wnt signaling pathway melanosome transport cilium assembly fat cell differentiation intermembrane transport protein polymerization protein localization to cilium protein localization to nonmotile primary cilium biological_process protein localization to ciliary membrane protein ADP-ribosylation transport Ras protein signal transduction protein transport cell projection organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BBS3 Polyclonal Antibody

BBS3 Antibody (12676-1-AP) in ICC/IF

Product Details

12676-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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