Proteintech

BCKDHB Polyclonal Antibody

BCKDHB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

BCKDHB Antibody (13685-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 13685-1-AP) (BCKDHB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

13685-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BCKDHB Fusion Protein Ag4552 (41-392 aa encoded by BC040139)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TVEDAAQRR QVAHFTFQPD PEPREYGQTQ KMNLFQSVTS ALDNSLAKDP TAVIFGEDVA FGGVFRCTVG LRDKYGKDRV FNTPLCEQGI VGFGIGIAVT GATAIAEIQF ADYIFPAFDQ IVNEAAKYRY RSGDLFNCGS LTIRSPWGCV GHGALYHSQS PEAFFAHCPG IKVVIPRSPF QAKGLLLSCI EDKNPCIFFE PKILYRAAAE EVPIEPYNIP LSQAEVIQEG SDVTLVAWGT QVHVIREVAS MAKEKLGVSC EVIDLRTIIP WDVDTICKSV IKTGRLLISH EAPLTGGFAS EISSTVQEEC FLNLEAPISR VCGYDTPFPH IFEPFYIPDK WKCYDALRKM INY (41-392 aa encoded by BC040139)

Target Information

Branched-chain keto acid dehydrogenase is a multienzyme complex associated with the inner membrane of mitochondria, and functions in the catabolism of branched-chain amino acids. The complex consists of multiple copies of 3 components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). This gene encodes the E1 beta subunit, and mutations therein have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation, and feeding problems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis.

Nature communications

Wang Y,Luo M,Wang F,Tong Y,Li L,Shu Y,Qiao K,Zhang L,Yan G,Liu J,Ji H,Xie Y,Zhang Y,Gao WQ,Liu Y

Thu Nov 24 00:00:00 UTC 2022

Branched-chain amino acid catabolism breaks glutamine addiction to sustain hepatocellular carcinoma progression.

Cell reports

Yang D,Liu H,Cai Y,Lu K,Zhong X,Xing S,Song W,Zhang Y,Ye L,Zhu X,Wang T,Zhang P,Li ST,Feng J,Jia W,Zhang H,Gao P

Tue Nov 22 00:00:00 UTC 2022

SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis.

Cell reports

Zaganjor E,Yoon H,Spinelli JB,Nunn ER,Laurent G,Keskinidis P,Sivaloganathan S,Joshi S,Notarangelo G,Mulei S,Chvasta MT,Tucker SA,Kalafut K,van de Ven RAH,Clish CB,Haigis MC

Tue Jul 13 00:00:00 UTC 2021

MiR-330-5p negatively regulates ovine preadipocyte differentiation by targeting branched-chain aminotransferase 2.

Animal science journal = Nihon chikusan Gakkaiho

Shi T,Yan X,Qiao L,Li B,Cheng L,Pan Y,Jing J,Cao N,Liu W

Fri Jun 01 00:00:00 UTC 2018

MiR-330-5p negatively regulates ovine preadipocyte differentiation by targeting branched-chain aminotransferase 2.

Animal science journal = Nihon chikusan Gakkaiho

Shi T,Yan X,Qiao L,Li B,Cheng L,Pan Y,Jing J,Cao N,Liu W

Fri Jun 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: 2-oxoisovalerate dehydrogenase beta subunit; 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial; BCKD BCKDH E1-beta; BCKDE1B; BCKDH E1-beta; branched chain alpha-ketoacid dehydrogenase E1-beta subunit; branched chain keto acid dehydrogenase E1 beta; branched chain keto acid dehydrogenase E1, beta polypeptide; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; dJ279A18.1; E1b-beta subunit of the branched-chain complex; testis secretory sperm-binding protein Li 240mP

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Gene Aliases: BCKDE1B; BCKDH E1-beta; BCKDHB; E1B

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UniProt ID: (Human) P21953, (Rat) P35738, (Mouse) Q6P3A8

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Entrez Gene ID: (Human) 594, (Rat) 29711, (Mouse) 12040

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Function(s)

alpha-ketoacid dehydrogenase activity 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity protein binding carboxy-lyase activity catalytic activity protein complex binding oxidoreductase activity

Process(es)

response to nutrient metabolic process branched-chain amino acid catabolic process response to glucocorticoid response to cAMP oxidation-reduction process cellular amino acid catabolic process glyoxylate metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BCKDHB Polyclonal Antibody

BCKDHB Antibody (13685-1-AP) in IHC (P)

Product Details

13685-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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