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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • BCL-2 Antibodies

          OriGene

          BCL2 Monoclonal Antibody (OTI10C7), TrueMAB™

          Advanced Verification
          View all (186) BCL-2 antibodies

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          Datasheet
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          Cite BCL2 Monoclonal Antibody (OTI10C7), TrueMAB™

          • Antibody Testing Data (6)
          • Advanced Verification (1)
          BCL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          BCL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          BCL2 Antibody (TA806589) in IHC (P)

          Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-BCL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806589) {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          BCL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BCL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BCL2 Antibody in Western Blot (WB)
          BCL2 Antibody in Western Blot (WB)
          BCL2 Antibody in Western Blot (WB)
          BCL2 Antibody in Western Blot (WB)
          BCL2 Antibody

          Product Details

          TA806589

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:150
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          OTI10C7

          Immunogen

          Full length human recombinant protein of human BCL2 produced in HEK293T cell.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 1% BSA, 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl 2; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

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          Gene Aliases: Bcl-2; BCL2; PPP1R50

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          UniProt ID: (Human) P10415

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          Entrez Gene ID: (Human) 596

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          Function(s)
          protease binding protein binding transcription factor binding channel activity channel inhibitor activity ubiquitin protein ligase binding identical protein binding protein homodimerization activity sequence-specific DNA binding protein heterodimerization activity BH3 domain binding protein phosphatase 2A binding
          Process(es)
          protein polyubiquitination ossification ovarian follicle development metanephros development branching involved in ureteric bud morphogenesis behavioral fear response B cell homeostasis release of cytochrome c from mitochondria regulation of cell-matrix adhesion lymphoid progenitor cell differentiation B cell lineage commitment response to ischemia renal system process protein dephosphorylation melanin metabolic process regulation of nitrogen utilization apoptotic process humoral immune response cellular response to DNA damage stimulus actin filament organization axonogenesis female pregnancy cell aging male gonad development extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway in response to oxidative stress response to radiation response to toxic substance post-embryonic development response to iron ion response to UV-B response to gamma radiation regulation of gene expression negative regulation of autophagy negative regulation of calcium ion transport into cytosol regulation of glycoprotein biosynthetic process mesenchymal cell development positive regulation of neuron maturation positive regulation of smooth muscle cell migration cell growth peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cochlear nucleus development gland morphogenesis regulation of transmembrane transporter activity negative regulation of ossification positive regulation of cell growth negative regulation of cell growth melanocyte differentiation negative regulation of cell migration positive regulation of B cell proliferation hair follicle morphogenesis axon regeneration regulation of protein stability endoplasmic reticulum calcium ion homeostasis glomerulus development negative regulation of cellular pH reduction negative regulation of myeloid cell apoptotic process T cell differentiation in thymus positive regulation of peptidyl-serine phosphorylation negative regulation of osteoblast proliferation response to cytokine response to nicotine organ growth positive regulation of multicellular organism growth B cell proliferation cellular response to glucose starvation response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process positive regulation of catalytic activity CD8-positive, alpha-beta T cell lineage commitment regulation of protein homodimerization activity regulation of protein heterodimerization activity negative regulation of neuron apoptotic process ear development regulation of viral genome replication positive regulation of melanocyte differentiation negative regulation of retinal cell programmed cell death regulation of mitochondrial membrane permeability focal adhesion assembly spleen development thymus development digestive tract morphogenesis oocyte development positive regulation of skeletal muscle fiber development pigment granule organization homeostasis of number of cells within a tissue B cell receptor signaling pathway response to glucocorticoid neuron apoptotic process defense response to virus regulation of mitochondrial membrane potential negative regulation of mitochondrial depolarization regulation of calcium ion transport transmembrane transport intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to organic substance cellular response to hypoxia reactive oxygen species metabolic process extrinsic apoptotic signaling pathway in absence of ligand positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway negative regulation of G1/S transition of mitotic cell cycle negative regulation of reactive oxygen species metabolic process negative regulation of anoikis negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.
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