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Invitrogen
BCL9 Polyclonal Antibody
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FIGURE: 1 / 10
BCL9 Antibody (PA5-100196) in ICC/IF
Immunofluorescent analysis of BCL9 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and BCL9 polyclonal antibody (Product # PA5-100196) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100196
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BCL9(Accession O00512), corresponding to amino acid residues I860-V910.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815726
Product Specific Information
Antibody detects endogenous levels of total BCL9.
Target Information
BCL9 is involved in signal transduction through the Wnt pathway. It promotes beta-catenin's transcriptional activity. BCL9 is associated with B-cell acute lymphoblastic leukemia. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell CLL/lymphoma 9 protein; B-cell lymphoma 9 protein; bcl-9; nuclear co-factor of beta-catenin signalling; Protein legless homolog
Gene Aliases: 2610202E01Rik; 8030475K17Rik; A330041G23Rik; BCL9; Gm130; LGS
UniProt ID: (Human) O00512, (Mouse) Q9D219
Entrez Gene ID: (Human) 607, (Mouse) 77578
myotube differentiation involved in skeletal muscle regeneration
Wnt signaling pathway
somatic stem cell population maintenance
skeletal muscle cell differentiation
positive regulation of transcription from RNA polymerase II promoter
canonical Wnt signaling pathway
regulation of transforming growth factor beta receptor signaling pathway
beta-catenin-TCF complex assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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