Proteintech

BDH1 Polyclonal Antibody

BDH1 Antibody in Immunocytochemistry (ICC/IF)

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BDH1 Antibody (15417-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15417-1-AP (BDH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

BDH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BDH1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15417-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BDH1 Fusion Protein Ag7705 (1-343 aa encoded by BC005844)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MLATRLSRP LSRLPGKTLS ACDRENGARR PLLLGSTSFI PIGRRTYASA AEPVGSKAVL VTGCDSGFGF SLAKHLHSKG FLVFAGCLMK DKGHDGVKEL DSLNSDRLRT VQLNVCSSEE VEKVVEIVRS SLKDPEKGMW GLVNNAGIST FGEVEFTSLE TYKQVAEVNL WGTVRMTKSF LPLIRRAKGR VVNISSMLGR MANPARSPYC ITKFGVEAFS DCLRYEMYPL GVKVSVVEPG NFIAATSLYS PESIQAIAKK MWEELPEVVR KDYGKKYFDE KIAKMETYCS SGSTDTSPVI DAVTHALTAT TPYTRYHPMD YYWWLRMQIM THLPGAISDM IYIR (1-343 aa encoded by BC005844)

Target Information

This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and -3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of hepatic phosphoenolpyruvate carboxykinase 1 dysregulates metabolic responses to acute exercise but enhances adaptations to exercise training in mice.

American journal of physiology. Endocrinology and metabolism

Rome FI,Shobert GL,Voigt WC,Stagg DB,Puchalska P,Burgess SC,Crawford PA,Hughey CC

Sun Jan 01 00:00:00 UTC 2023

Ketogenic diet modulates cardiac metabolic dysregulation in streptozocin-induced diabetic rats.

The Journal of nutritional biochemistry

Trang NN,Lee TW,Kao YH,Chao TF,Lee TI,Chen YJ

Sun Jan 01 00:00:00 UTC 2023

Metabolism-Based Molecular Subtyping Endows Effective Ketogenic Therapy in p53-Mutant Colon Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Tang M,Xu H,Huang H,Kuang H,Wang C,Li Q,Zhang X,Ge Y,Song M,Zhang X,Wang Z,Ma C,Kang J,Zhang W,Wang Y,Zhang B,Zhang X,Chen Y,Cong M,Melino G,Wang X,Zhou F,Sun Q,Shi H

Sat Oct 01 00:00:00 UTC 2022

Metabolism-Based Molecular Subtyping Endows Effective Ketogenic Therapy in p53-Mutant Colon Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Tang M,Xu H,Huang H,Kuang H,Wang C,Li Q,Zhang X,Ge Y,Song M,Zhang X,Wang Z,Ma C,Kang J,Zhang W,Wang Y,Zhang B,Zhang X,Chen Y,Cong M,Melino G,Wang X,Zhou F,Sun Q,Shi H

Sat Oct 01 00:00:00 UTC 2022

Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.

Frontiers in oncology

Ye T,Lin L,Cao L,Huang W,Wei S,Shan Y,Zhang Z

Sat Jul 16 00:00:00 UTC 2022

Ketogenic diet uncovers differential metabolic plasticity of brain cells.

Science advances

Düking T,Spieth L,Berghoff SA,Piepkorn L,Schmidke AM,Mitkovski M,Kannaiyan N,Hosang L,Scholz P,Shaib AH,Schneider LV,Hesse D,Ruhwedel T,Sun T,Linhoff L,Trevisiol A,Köhler S,Pastor AM,Misgeld T,Sereda M,Hassouna I,Rossner MJ,Odoardi F,Ischebeck T,de Hoz L,Hirrlinger J,Jahn O,Saher G

Fri Sep 16 00:00:00 UTC 2022

Ketone body β-hydroxybutyrate ameliorates colitis by promoting M2 macrophage polarization through the STAT6-dependent signaling pathway.

BMC medicine

Huang C,Wang J,Liu H,Huang R,Yan X,Song M,Tan G,Zhi F

Fri Apr 15 00:00:00 UTC 2022

Disrupted liver oxidative metabolism in glycine N-methyltransferase-deficient mice is mitigated by dietary methionine restriction.

Molecular metabolism

Rome FI,Hughey CC

Fri Apr 01 00:00:00 UTC 2022

β-hydroxybutyrate reduces reinstatement of cocaine conditioned place preference through hippocampal CaMKII-α β-hydroxybutyrylation.

Cell reports

Li H,Wan X,Wu Z,Zhou Y,Chen R,Xu W,Zhang J,Yang Z,Bai L,Zhang J,Qin F,Wang L,Chen Y,Jiang L,He Y,Wang X,Wei Q,Li S,Dai Y,Chen Y,Wang Y,Wang H,Tian J,Zhao Y,Cen X

Tue Nov 29 00:00:00 UTC 2022

Ketone body oxidation increases cardiac endothelial cell proliferation.

EMBO molecular medicine

Weis EM,Puchalska P,Nelson AB,Taylor J,Moll I,Hasan SS,Dewenter M,Hagenmüller M,Fleming T,Poschet G,Hotz-Wagenblatt A,Backs J,Crawford PA,Fischer A

Thu Apr 07 00:00:00 UTC 2022

Maternal use of methamphetamine induces sex-dependent changes in myocardial gene expression in adult offspring.

Physiological reports

Dague A,Chavva H,Brazeau DA,Denvir J,Rorabaugh BR

Tue Nov 01 00:00:00 UTC 2022

Deficiency of 3-hydroxybutyrate dehydrogenase (BDH1) in mice causes low ketone body levels and fatty liver during fasting.

Journal of inherited metabolic disease

Otsuka H,Kimura T,Ago Y,Nakama M,Aoyama Y,Abdelkreem E,Matsumoto H,Ohnishi H,Sasai H,Osawa M,Yamaguchi S,Mitchell GA,Fukao T

Tue Sep 01 00:00:00 UTC 2020

Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.

Cell metabolism

Puchalska P,Martin SE,Huang X,Lengfeld JE,Daniel B,Graham MJ,Han X,Nagy L,Patti GJ,Crawford PA

Tue Feb 05 00:00:00 UTC 2019

Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.

Cell metabolism

Puchalska P,Martin SE,Huang X,Lengfeld JE,Daniel B,Graham MJ,Han X,Nagy L,Patti GJ,Crawford PA

Tue Feb 05 00:00:00 UTC 2019

The failing heart utilizes 3-hydroxybutyrate as a metabolic stress defense.

JCI insight

Horton JL,Davidson MT,Kurishima C,Vega RB,Powers JC,Matsuura TR,Petucci C,Lewandowski ED,Crawford PA,Muoio DM,Recchia FA,Kelly DP

Thu Feb 21 00:00:00 UTC 2019

Fenofibrate prevents skeletal muscle loss in mice with lung cancer.

Proceedings of the National Academy of Sciences of the United States of America

Goncalves MD,Hwang SK,Pauli C,Murphy CJ,Cheng Z,Hopkins BD,Wu D,Loughran RM,Emerling BM,Zhang G,Fearon DT,Cantley LC

Tue Jan 23 00:00:00 UTC 2018

Fenofibrate prevents skeletal muscle loss in mice with lung cancer.

Proceedings of the National Academy of Sciences of the United States of America

Goncalves MD,Hwang SK,Pauli C,Murphy CJ,Cheng Z,Hopkins BD,Wu D,Loughran RM,Emerling BM,Zhang G,Fearon DT,Cantley LC

Tue Jan 23 00:00:00 UTC 2018

Ketone body 3-hydroxybutyrate mimics calorie restriction via the Nrf2 activator, fumarate, in the retina.

Aging cell

Izuta Y,Imada T,Hisamura R,Oonishi E,Nakamura S,Inagaki E,Ito M,Soga T,Tsubota K

Thu Feb 01 00:00:00 UTC 2018

Ketone body 3-hydroxybutyrate mimics calorie restriction via the Nrf2 activator, fumarate, in the retina.

Aging cell

Izuta Y,Imada T,Hisamura R,Oonishi E,Nakamura S,Inagaki E,Ito M,Soga T,Tsubota K

Thu Feb 01 00:00:00 UTC 2018

Low ketolytic enzyme levels in tumors predict ketogenic diet responses in cancer cell lines in vitro and in vivo.

Journal of lipid research

Zhang J,Jia PP,Liu QL,Cong MH,Gao Y,Shi HP,Yu WN,Miao MY

Sun Apr 01 00:00:00 UTC 2018

Low ketolytic enzyme levels in tumors predict ketogenic diet responses in cancer cell lines in vitro and in vivo.

Journal of lipid research

Zhang J,Jia PP,Liu QL,Cong MH,Gao Y,Shi HP,Yu WN,Miao MY

Sun Apr 01 00:00:00 UTC 2018

Low ketolytic enzyme levels in tumors predict ketogenic diet responses in cancer cell lines in vitro and in vivo.

Journal of lipid research

Zhang J,Jia PP,Liu QL,Cong MH,Gao Y,Shi HP,Yu WN,Miao MY

Sun Apr 01 00:00:00 UTC 2018

Low ketolytic enzyme levels in tumors predict ketogenic diet responses in cancer cell lines in vitro and in vivo.

Journal of lipid research

Zhang J,Jia PP,Liu QL,Cong MH,Gao Y,Shi HP,Yu WN,Miao MY

Sun Apr 01 00:00:00 UTC 2018

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.

The Journal of cell biology

Schatton D,Pla-Martin D,Marx MC,Hansen H,Mourier A,Nemazanyy I,Pessia A,Zentis P,Corona T,Kondylis V,Barth E,Schauss AC,Velagapudi V,Rugarli EI

Mon Mar 06 00:00:00 UTC 2017

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.

The Journal of cell biology

Schatton D,Pla-Martin D,Marx MC,Hansen H,Mourier A,Nemazanyy I,Pessia A,Zentis P,Corona T,Kondylis V,Barth E,Schauss AC,Velagapudi V,Rugarli EI

Mon Mar 06 00:00:00 UTC 2017

Nutritional Ketosis Affects Metabolism and Behavior in Sprague-Dawley Rats in Both Control and Chronic Stress Environments.

Frontiers in molecular neuroscience

Brownlow ML,Jung SH,Moore RJ,Bechmann N,Jankord R

Tue Sep 29 00:00:00 UTC 2020

Nutritional Ketosis Affects Metabolism and Behavior in Sprague-Dawley Rats in Both Control and Chronic Stress Environments.

Frontiers in molecular neuroscience

Brownlow ML,Jung SH,Moore RJ,Bechmann N,Jankord R

Tue Sep 29 00:00:00 UTC 2020

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Cell research

Huang D,Li T,Wang L,Zhang L,Yan R,Li K,Xing S,Wu G,Hu L,Jia W,Lin SC,Dang CV,Song L,Gao P,Zhang H

Sat Oct 01 00:00:00 UTC 2016

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Cell research

Huang D,Li T,Wang L,Zhang L,Yan R,Li K,Xing S,Wu G,Hu L,Jia W,Lin SC,Dang CV,Song L,Gao P,Zhang H

Sat Oct 01 00:00:00 UTC 2016

Impairments of hepatic gluconeogenesis and ketogenesis in PPARα-deficient neonatal mice.

American journal of physiology. Endocrinology and metabolism

Cotter DG,Ercal B,d'Avignon DA,Dietzen DJ,Crawford PA

Tue Jul 15 00:00:00 UTC 2014

Impairments of hepatic gluconeogenesis and ketogenesis in PPARα-deficient neonatal mice.

American journal of physiology. Endocrinology and metabolism

Cotter DG,Ercal B,d'Avignon DA,Dietzen DJ,Crawford PA

Tue Jul 15 00:00:00 UTC 2014

Impact of peripheral ketolytic deficiency on hepatic ketogenesis and gluconeogenesis during the transition to birth.

The Journal of biological chemistry

Cotter DG,Ercal B,d'Avignon DA,Dietzen DJ,Crawford PA

Fri Jul 05 00:00:00 UTC 2013

Impact of peripheral ketolytic deficiency on hepatic ketogenesis and gluconeogenesis during the transition to birth.

The Journal of biological chemistry

Cotter DG,Ercal B,d'Avignon DA,Dietzen DJ,Crawford PA

Fri Jul 05 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: (R)-3-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase (heart, mitochondrial); b-hydroxybutyrate dehydrogenase; BDH; D-beta-hydroxybutyrate dehydrogenase, mitochondrial; MGC2723; MGC4347; MGC9788; Short chain dehydrogenase/reductase family 9C member 1; short chain dehydrogenase/reductase family 9C, member 1

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Gene Aliases: 2310032J20Rik; AI327223; BDH; BDH1; SDR9C1

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UniProt ID: (Human) Q02338, (Rat) P29147, (Mouse) Q80XN0

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Entrez Gene ID: (Human) 622, (Rat) 117099, (Mouse) 71911

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Function(s)

3-hydroxybutyrate dehydrogenase activity phospholipid binding catalytic activity oxidoreductase activity

Process(es)

liver development brain development response to nutrient response to toxic substance response to estradiol response to insulin response to drug response to starvation response to ethanol response to cadmium ion ketone body biosynthetic process ketone body catabolic process response to corticosterone oxidation-reduction process response to growth hormone adipose tissue development response to hormone metabolic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BDH1 Polyclonal Antibody

BDH1 Antibody (15417-1-AP) in ICC/IF

Product Details

15417-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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