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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • BDNF Antibodies

          Proteintech

          BDNF Polyclonal Antibody

          100 References
          View all (46) BDNF antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite BDNF Polyclonal Antibody

          • Antibody Testing Data (7)
          BDNF Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          BDNF Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          BDNF Antibody (28205-1-AP) in ICC/IF

          Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 28205-1-AP (BDNF antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          BDNF Antibody in Immunocytochemistry (ICC/IF)
          BDNF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BDNF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BDNF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BDNF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BDNF Antibody in Western Blot (WB)
          BDNF Antibody in Western Blot (WB)

          Product Details

          28205-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          View 82 publications 82 publications

          Immunohistochemistry (IHC)

          -
          View 5 publications 5 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 10 publications 10 publications

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication

          Not applicable (N/A)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          BDNF Fusion Protein Ag28276 (129-247 aa encoded by BC029795)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.33 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: HSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR (129-247 aa encoded by BC029795)

          Target Information

          BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Abrineurin; anorexia BDNF; brain derived neurothrophic factor; brain derived neurotrophic factor; Brain-derived neurotrophic factor; H-BDNF; Neurotrophic factor BDNF precursor form; neurotrophin; proBDNF

          View more View less

          Gene Aliases: ANON2; BDNF; BULN2

          View more View less

          UniProt ID: (Human) P23560, (Rat) P23363, (Mouse) P21237

          View more View less

          Entrez Gene ID: (Human) 627, (Rat) 24225, (Mouse) 12064

          View more View less

          Function(s)
          neurotrophin TRKB receptor binding growth factor activity receptor binding protein binding
          Process(es)
          ureteric bud development behavioral fear response response to hypoxia chronic inflammatory response mitochondrial electron transport, NADH to ubiquinone transmembrane receptor protein tyrosine kinase signaling pathway gamma-aminobutyric acid signaling pathway nervous system development negative regulation of neuroblast proliferation axon guidance axon target recognition learning or memory learning circadian rhythm feeding behavior neuron recognition response to light stimulus response to light intensity response to hormone response to auditory stimulus glutamate secretion response to fluoxetine response to activity dendrite development regulation of metabolic process nerve development neuron differentiation regulation of axon extension response to nutrient levels response to food negative regulation of synaptic transmission, GABAergic positive regulation of peptidyl-serine phosphorylation response to vitamin A response to tumor necrosis factor response to potassium ion mechanoreceptor differentiation response to drug fear response negative regulation of apoptotic process regulation of neuron apoptotic process negative regulation of neuron apoptotic process positive regulation of neuron differentiation negative regulation of striated muscle tissue development regulation of retinal cell programmed cell death regulation of synaptic plasticity regulation of long-term neuronal synaptic plasticity positive regulation of long-term neuronal synaptic plasticity regulation of short-term neuronal synaptic plasticity axon extension inner ear development cognition positive regulation of sequence-specific DNA binding transcription factor activity response to electrical stimulus positive regulation of synapse assembly response to hyperoxia excitatory postsynaptic potential inhibitory postsynaptic potential negative regulation of cell death taste bud development cellular response to tumor necrosis factor cellular response to norepinephrine stimulus response to anesthetic dendrite extension negative regulation of neuron death response to L-glutamate response to nerve growth factor cellular response to nerve growth factor stimulus neuron projection extension positive regulation of glucocorticoid receptor signaling pathway cell-cell signaling neuron projection morphogenesis cellular response to acid chemical synapse assembly brain-derived neurotrophic factor receptor signaling pathway positive regulation of brain-derived neurotrophic factor receptor signaling pathway collateral sprouting positive regulation of collateral sprouting
          It has to be done as per old AB suggested Products section.
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