Invitrogen

BET1 Polyclonal Antibody

4 Published Figures

4 References

View all (2) BET1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

FIGURE: 1 / 5

BET1 Antibody (PA5-88961) in WB

Western blot analysis of BET1 in extracts of various cell lines. Samples were incubated with BET1 Polyclonal antibody (Product # PA5-88961) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

BET1 Antibody in Immunocytochemistry, Immunoprecipitation (ICC/IF, IP)

BET1 Antibody in Immunocytochemistry (ICC/IF)

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Product Details

PA5-88961

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

1 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human BET1 (NP_0058591)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.83 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2805256

Product Specific Information

Immunogen sequence: MRRAGLGEGV PPGNYGNYGY ANSGYSACEE ENERLTESLR SKVTAIKSLS IEIGHEVKTQ NKLLAEMDSQ FDSTTGFLGK TMGKLKILSR GSQT; Positive Samples: U-87MG, HeLa; Cellular Location: Endoplasmic reticulum membrane, Golgi apparatus, Golgi apparatus membrane, Single-pass type IV membrane protein, cis-Golgi network membrane

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Microscopic and Biochemical Hallmarks of BICD2-Associated Muscle Pathology toward the Evaluation of Novel Variants.

International journal of molecular sciences

Unger A,Roos A,Gangfuß A,Hentschel A,Gläser D,Krause K,Doering K,Schara-Schmidt U,Hoffjan S,Vorgerd M,Güttsches AK

PA5-88961 was used in Immunohistochemistry (Frozen) to present one patient with a known and six patients with novel BICD2 missense variants, further characterizing the molecular landscape of this heterogenous neurological disorder.

Thu Apr 06 00:00:00 UTC 2023

SNARE protein USE1 is involved in the glycosylation and the expression of mumps virus fusion protein and important for viral propagation.

PLoS pathogens

Liu Y,Katoh H,Sekizuka T,Bae C,Wakata A,Kato F,Sakata M,Yamaji T,Wang Z,Takeda M

PA5-88961 was used in Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry to find that a SNARE subfamily protein, USE1, bound specifically to the MuV fusion (F) protein and was important for MuV propagation.

Thu Dec 01 00:00:00 UTC 2022

SNARE protein USE1 is involved in the glycosylation and the expression of mumps virus fusion protein and important for viral propagation.

PLoS pathogens

Liu Y,Katoh H,Sekizuka T,Bae C,Wakata A,Kato F,Sakata M,Yamaji T,Wang Z,Takeda M

Thu Dec 01 00:00:00 UTC 2022

SNARE protein USE1 is involved in the glycosylation and the expression of mumps virus fusion protein and important for viral propagation.

PLoS pathogens

Liu Y,Katoh H,Sekizuka T,Bae C,Wakata A,Kato F,Sakata M,Yamaji T,Wang Z,Takeda M

Thu Dec 01 00:00:00 UTC 2022

SNARE protein USE1 is involved in the glycosylation and the expression of mumps virus fusion protein and important for viral propagation.

PLoS pathogens

Liu Y,Katoh H,Sekizuka T,Bae C,Wakata A,Kato F,Sakata M,Yamaji T,Wang Z,Takeda M

Thu Dec 01 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: BET1 homolog; Bet1p homolog; blocked early in transport 1 homolog; Golgi vesicular membrane trafficking protein p18; Golgi vesicular membrane-trafficking protein p18; hBET1

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Gene Aliases: BET1; HBET1

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UniProt ID: (Human) O15155

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Entrez Gene ID: (Human) 10282

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Function(s)

protein binding syntaxin binding

Process(es)

ER to Golgi vesicle-mediated transport protein transport COPII vesicle coating vesicle fusion with Golgi apparatus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BET1 Polyclonal Antibody

BET1 Antibody (PA5-88961) in WB

Product Details

PA5-88961

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Bioinformatics

Performance Guarantee

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