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Invitrogen
BHLHB5 Polyclonal Antibody
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FIGURE: 1 / 3
BHLHB5 Antibody (PA5-100405) in ICC/IF
Immunocytochemistry analysis of BHLHB5 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with BHLHB5 polyclonal antibody (Product # PA5-100405) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100405
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BHLHE22(Accession Q8NFJ8), corresponding to amino acid residues L119-A169.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849917
Product Specific Information
Antibody detects endogenous levels of total BHLHE22.
Target Information
BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: basic helix-loop-helix (bHLH) gene, class B, beta3; basic helix-loop-helix domain containing, class B, 5; basic helix-loop-helix domain containing, class B5; basic helix-loop-helix family, member e22; bHLHb5; bHLHe22; Class B basic helix-loop-helix protein 5; Class E basic helix-loop-helix protein 22; Protein BETA3; trinucleotide repeat containing 20; Trinucleotide repeat-containing gene 20 protein
Gene Aliases: Beta3; BHLHB5; BHLHE22; CAGL85; TNRC20
UniProt ID: (Human) Q8NFJ8, (Mouse) Q8C6A8
Entrez Gene ID: (Human) 27319, (Mouse) 59058, (Rat) 365748
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
neurogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
corpus callosum morphogenesis
cerebral cortex regionalization
central nervous system projection neuron axonogenesis
corticospinal tract morphogenesis
anterior commissure morphogenesis
neuron differentiation
negative regulation of transcription, DNA-templated
retinal bipolar neuron differentiation
retina morphogenesis in camera-type eye
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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