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BHLHE40 Antibody (PA1-16546) in WB
Western blot analysis of BHLHE40 in whole cell protein from A431 and PC3. Samples were incubated in BHLHE40 polyclonal antibody (Product # PA1-16546) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. A431 (lane 1) and PC3 (lane 2) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.
Product Details
PA1-16546
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Residues between 350 and the C-terminus (412) of human DEC1 using the numbering given in entry NP_003661.1 (GeneID 8553)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2065348
Product Specific Information
Suggested positive control: antigen standard for BHLHB2 (transient overexpression lysate).
Target Information
Differentiated embryo-chondrocyte expressed gene 1 (DEC1) is an important protein involved in embryonic cell differentiation, proliferation, and apoptosis. It has also recently been shown to be regulated by hypoxia. The loss of DEC1 expression may be an early event in the development of lung cancer, while DEC1 may be up-regulated by hypoxia in other cancers and in more extreme hypoxia it may have a role in cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1-Dec; basic helix-loop-helix domain containing, class B, 2; basic helix-loop-helix domain containing, class B2; basic helix-loop-helix family, member e40; bHLHb2; bHLHe40; Class B basic helix-loop-helix protein 2; Class E basic helix-loop-helix protein 40; cytokine response gene 8; DEC1; differentially expressed in chondrocytes 1; Differentially expressed in chondrocytes protein 1; differentiated embryo chondrocyte expressed gene 1; E47 interaction protein 1; EIP1; eip1 (E47 interaction protein 1); enhancer-of split and hairy-related protein 2; Enhancer-of-split and hairy-related protein 2; FLJ99214; SHARP-2; stimulated by retinoic acid 14; Stimulated by retinoic acid gene 13 protein
Gene Aliases: BHLHB2; BHLHE40; C130042M06Rik; Clast5; CR8; DEC1; HLHB2; SHARP-2; SHARP2; STRA13; Stra14
UniProt ID: (Human) O14503, (Mouse) O35185, (Rat) O35780
Entrez Gene ID: (Human) 8553, (Mouse) 20893, (Rat) 79431
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
transcriptional repressor activity, RNA polymerase II transcription factor binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription corepressor activity
protein binding
protein domain specific binding
protein homodimerization activity
bHLH transcription factor binding
MRF binding
protein heterodimerization activity
E-box binding
DNA binding
protein dimerization activity
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
response to light stimulus
entrainment of circadian clock
circadian regulation of gene expression
entrainment of circadian clock by photoperiod
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of transcription, DNA-templated
rhythmic process
nervous system development
circadian rhythm
regulation of neuronal synaptic plasticity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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