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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • BID Antibodies

          OriGene

          BID Monoclonal Antibody (OTI3E9), TrueMAB™

          View all (53) BID antibodies

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          Datasheet
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          Cite BID Monoclonal Antibody (OTI3E9), TrueMAB™

          • Antibody Testing Data (7)
          BID Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          BID Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          BID Antibody (CF506766) in ICC/IF

          Anti-BID mouse monoclonal antibody (TA506766) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BID(RC207261). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          BID Antibody in Immunocytochemistry (ICC/IF)
          BID Antibody in Immunocytochemistry (ICC/IF)
          BID Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BID Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BID Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          BID Antibody in Western Blot (WB)
          BID Antibody in Western Blot (WB)

          Product Details

          CF506766

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:400-1:4,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:150
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          OTI3E9

          Immunogen

          Full length human recombinant protein of human BID produced in HEK293T cell.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 8% trehalose

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

          Target Information

          BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: apoptic d; apoptic death agonist; BH3-interacting domain death agonist; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p22 BID

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          Gene Aliases: 2700049M22Rik; AI875481; AU022477; BID; FP497

          View more View less

          UniProt ID: (Human) P55957, (Mouse) P70444

          View more View less

          Entrez Gene ID: (Human) 637, (Mouse) 12122

          View more View less

          Function(s)
          death receptor binding protein binding ubiquitin protein ligase binding
          Process(es)
          release of cytochrome c from mitochondria protein targeting to mitochondrion activation of cysteine-type endopeptidase activity involved in apoptotic process brain development extrinsic apoptotic signaling pathway via death domain receptors apoptotic mitochondrial changes response to estradiol positive regulation of protein oligomerization positive regulation of protein homooligomerization glial cell apoptotic process regulation of cell proliferation signal transduction in response to DNA damage regulation of apoptotic process positive regulation of apoptotic process protein homooligomerization neuron apoptotic process establishment of protein localization to membrane positive regulation of release of cytochrome c from mitochondria hepatocyte apoptotic process mitochondrial outer membrane permeabilization positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway regulation of G1/S transition of mitotic cell cycle positive regulation of extrinsic apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway apoptotic process regulation of protein oligomerization extrinsic apoptotic signaling pathway regulation of mitochondrial membrane permeability involved in apoptotic process
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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