Proteintech

BIN1 Polyclonal Antibody

BIN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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BIN1 Antibody (14647-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue slide using (Product # 14647-1-AP) BIN1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BIN1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

BIN1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14647-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BIN1 Fusion Protein Ag6240 (1-439 aa encoded by BC004101)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAEMGSKGV TAGKIASNVQ KKLTRAQEKV LQKLGKADET KDEQFEQCVQ NFNKQLTEGT RLQKDLRTYL ASVKAMHEAS KKLNECLQEV YEPDWPGRDE ANKIAENNDL LWMDYHQKLV DQALLTMDTY LGQFPDIKSR IAKRGRKLVD YDSARHHYES LQTAKKKDEA KIAKAEEELI KAQKVFEEMN VDLQEELPSL WNSRVGFYVN TFQSIAGLEE NFHKEMSKLN QNLNDVLVGL EKQHGSNTFT VKAQPSDNAP AKGNKSPSPP DGSPAATPEI RVNHEPEPAG GATPGATLPK SPSQPAEASE VAGGTQPAAG AQEPGETAAS EAASSSLPAV VVETFPATVN GTVEGGSGAG RLDLPPGFMF KVQAQHDYTA TDTDELQLRA GDVVLVIPFQ NPEEQDEGWL MGVKESDWNQ HKELEKCRGV FPENFTERVP (1-439 aa encoded by BC004101)

Target Information

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The role of Limch1 alternative splicing in skeletal muscle function.

Life science alliance

Penna MS,Hu RC,Rodney GG,Cooper TA

Thu Jun 01 00:00:00 UTC 2023

Small and Large Extracellular Vesicles Derived from Pleural Mesothelioma Cell Lines Offer Biomarker Potential.

Cancers

Ahmadzada T,Vijayan A,Vafaee F,Azimi A,Reid G,Clarke S,Kao S,Grau GE,Hosseini-Beheshti E

Tue Apr 18 00:00:00 UTC 2023

BIN1 is a key regulator of proinflammatory and neurodegeneration-related activation in microglia.

Molecular neurodegeneration

Sudwarts A,Ramesha S,Gao T,Ponnusamy M,Wang S,Hansen M,Kozlova A,Bitarafan S,Kumar P,Beaulieu-Abdelahad D,Zhang X,Collier L,Szekeres C,Wood LB,Duan J,Thinakaran G,Rangaraju S

Sat May 07 00:00:00 UTC 2022

Long non-coding RNA SNHG10 upregulates BIN1 to suppress the tumorigenesis and epithelial-mesenchymal transition of epithelial ovarian cancer via sponging miR-200a-3p.

Cell death discovery

Lv W,Jia Y,Wang J,Duan Y,Wang X,Liu T,Hao S,Liu L

Fri Feb 11 00:00:00 UTC 2022

Long non-coding RNA SNHG10 upregulates BIN1 to suppress the tumorigenesis and epithelial-mesenchymal transition of epithelial ovarian cancer via sponging miR-200a-3p.

Cell death discovery

Lv W,Jia Y,Wang J,Duan Y,Wang X,Liu T,Hao S,Liu L

Fri Feb 11 00:00:00 UTC 2022

Combining Hypothermia and Oleuropein Subacutely Protects Subcortical White Matter in a Swine Model of Neonatal Hypoxic-Ischemic Encephalopathy.

Journal of neuropathology and experimental neurology

Lee JK,Santos PT,Chen MW,O'Brien CE,Kulikowicz E,Adams S,Hardart H,Koehler RC,Martin LJ

Wed Jan 20 00:00:00 UTC 2021

Fractional anisotropy from diffusion tensor imaging correlates with acute astrocyte and myelin swelling in neonatal swine models of excitotoxic and hypoxic-ischemic brain injury.

The Journal of comparative neurology

Lee JK,Liu D,Jiang D,Kulikowicz E,Tekes A,Liu P,Qin Q,Koehler RC,Aggarwal M,Zhang J,Martin LJ

Thu Jul 01 00:00:00 UTC 2021

Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation.

Cell reports

De Rossi P,Nomura T,Andrew RJ,Masse NY,Sampathkumar V,Musial TF,Sudwarts A,Recupero AJ,Le Metayer T,Hansen MT,Shim HN,Krause SV,Freedman DJ,Bindokas VP,Kasthuri N,Nicholson DA,Contractor A,Thinakaran G

Tue Mar 10 00:00:00 UTC 2020

Neuronal BIN1 Regulates Presynaptic Neurotransmitter Release and Memory Consolidation.

Cell reports

Tue Mar 10 00:00:00 UTC 2020

Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.

Toxicology and applied pharmacology

Ebokaiwe AP,Njoya EM,Sheng Y,Zhang Z,Li S,Zhou Z,Qiang Z,Peng T,Hussein AA,Zhang G,Lu X,Li L,Wang F

Thu Oct 01 00:00:00 UTC 2020

Reduction of the expression of the late-onset Alzheimer's disease (AD) risk-factor BIN1 does not affect amyloid pathology in an AD mouse model.

The Journal of biological chemistry

Andrew RJ,De Rossi P,Nguyen P,Kowalski HR,Recupero AJ,Guerbette T,Krause SV,Rice RC,Laury-Kleintop L,Wagner SL,Thinakaran G

Fri Mar 22 00:00:00 UTC 2019

Reduction of the expression of the late-onset Alzheimer's disease (AD) risk-factor BIN1 does not affect amyloid pathology in an AD mouse model.

The Journal of biological chemistry

Andrew RJ,De Rossi P,Nguyen P,Kowalski HR,Recupero AJ,Guerbette T,Krause SV,Rice RC,Laury-Kleintop L,Wagner SL,Thinakaran G

Fri Mar 22 00:00:00 UTC 2019

Low expression of Bin1, along with high expression of IDO in tumor tissue and draining lymph nodes, are predictors of poor prognosis for esophageal squamous cell cancer patients.

International journal of cancer

Jia Y,Wang H,Wang Y,Wang T,Wang M,Ma M,Duan Y,Meng X,Liu L

Tue Sep 01 00:00:00 UTC 2015

Low expression of Bin1, along with high expression of IDO in tumor tissue and draining lymph nodes, are predictors of poor prognosis for esophageal squamous cell cancer patients.

International journal of cancer

Jia Y,Wang H,Wang Y,Wang T,Wang M,Ma M,Duan Y,Meng X,Liu L

Tue Sep 01 00:00:00 UTC 2015

Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.

Molecular biology of the cell

Giridharan SS,Cai B,Vitale N,Naslavsky N,Caplan S

Sat Jun 01 00:00:00 UTC 2013

Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.

Molecular biology of the cell

Giridharan SS,Cai B,Vitale N,Naslavsky N,Caplan S

Sat Jun 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: AMPH 2; Amph2; amphiphysin 2; Amphiphysin II; amphiphysin IIamph2; amphiphysin-like; Amphiphysin-like protein; box dependant MYC interacting protein 1; Box-dependent myc-interacting protein 1; Bridging integrator 1; DKFZp547F068; MGC10367; MGC105358; myc box dependent interacting protein 1; Myc box-dependent-interacting protein 1; SH3 domain-containing protein 9

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Gene Aliases: ALP-1; AMPH2; AMPHL; BIN1; BRAMP-2; SH3P9

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UniProt ID: (Human) O00499, (Mouse) O08539, (Rat) O08839

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Entrez Gene ID: (Human) 274, (Mouse) 30948, (Rat) 117028

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Function(s)

protein binding identical protein binding tau protein binding actin filament binding RNA polymerase binding protein complex binding protein heterodimerization activity GTPase binding

Process(es)

endocytosis multicellular organism development regulation of endocytosis cell differentiation muscle cell differentiation positive regulation of apoptotic process positive regulation of GTPase activity regulation of neuron differentiation positive regulation of endocytosis positive regulation of astrocyte differentiation lipid tube assembly regulation of cell cycle arrest nucleus organization cell proliferation viral process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BIN1 Polyclonal Antibody

BIN1 Antibody (14647-1-AP) in IHC (P)

Product Details

14647-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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