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Invitrogen
BLOS1 Polyclonal Antibody
FIGURE: 1 / 2
BLOS1 Antibody (PA5-109347) in IHC (P)
Immunohistochemistry analysis of BLOS1 in paraffin-embedded A. thaliana tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with BLOS1 polyclonal antibody (Product # PA5-109347) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
Product Details
PA5-109347
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within thaliana BLOS1 aa 26-75
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854758
Product Specific Information
Positive Control: Rice tissue lysates, A. thaliana tissue.
Target Information
Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1), a complex that mediates the vacuolar degradative transport via the intracellular vesicle trafficking from the endosome to the vacuole. Probably regulates the PIN1 and PIN2 homeostasis through its interaction with SNX1. An eight-subunit protein complex, BLOC-1, which is involved in endosomal transport from the endosomes to the lysosomes, has been identified in both human and mice. In this study, two homologous subunits of this complex, BLOS1 (or AtGCN5L1) and BLOS2, have been characterized in Arabidopsis. Both BLOS1 and BLOS2 interacted with SNX1 on the sorting endosomes. Inducible RNAi lines with reduced levels of BLOS1 had longer primary roots and more lateral roots. Consistently, PIN1 and PIN2 were increased in BLOS1 RNAi lines, implicating an impaired transport from the endosomes to the vacuoles. These results suggest that a putative BLOC-1 complex in Arabidopsis might mediate the vacuolar degradative transport through direct interaction with SNX1 to regulate the homeostasis of PIN1 and PIN2, which is important for plant growth and development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Biogenesis of lysosome-related organelles complex 1 subunit 1; BLOC-1 subunit 1; GCN5-like protein 1; Protein RT14 homolog
Gene Aliases: AT2G30330; BLOC subunit 1; BLOS1; GCN5L1; T09D09.14; T9D9.14; T9D9_14
UniProt ID: (Arabidopsis) O22929
Entrez Gene ID: (Arabidopsis) 817583
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