Invitrogen

BMI-1 Monoclonal Antibody (3E3)

BMI-1 Antibody in Immunocytochemistry (ICC/IF)

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BMI-1 Antibody (MA5-15919) in ICC/IF

Immunofluorescence analysis of HeLa cells using BMI1 monoclonal antibody (Product # MA5-15919) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

BMI-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-15919

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3E3

Immunogen

Purified recombinant fragment of human BMI1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11152159

Product Specific Information

MA5-15919 targets BMI1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15919 immunogen is purified recombinant fragment of human BMI1 expressed in E. Coli. .

MA5-15919 detects BMI1 which has a predicted molecular weight of approximately 37kDa.

Target Information

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BMI1 maintains the Treg epigenomic landscape to prevent inflammatory bowel disease.

The Journal of clinical investigation

Gonzalez MM,Bamidele AO,Svingen PA,Sagstetter MR,Smyrk TC,Gaballa JM,Hamdan FH,Kosinsky RL,Gibbons HR,Sun Z,Ye Z,Nair A,Ramos GP,Braga Neto MB,Wixom AQ,Mathison AJ,Johnsen SA,Urrutia R,Faubion WA

MA5-15919 was used in Proximity Ligation Assay (PLA) to investigate the role of polycomb repressive complexes in FOXP3 target gene regulation.

Tue Jun 15 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: B lymphoma Mo-MLV insertion region 1; B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; murine leukemia viral (bmi-1) oncogene homolog; Pcgf4 antibody; Polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; Polycomb group RING finger protein 4; RING finger protein 51; RP11-573G6.1

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Gene Aliases: AW546694; Bmi-1; BMI1; flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51

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UniProt ID: (Human) P35226, (Mouse) P25916

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Entrez Gene ID: (Human) 648, (Mouse) 12151

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Function(s)

protein binding zinc ion binding RING-like zinc finger domain binding promoter-specific chromatin binding chromatin binding sequence-specific DNA binding metal ion binding ubiquitin-protein transferase activator activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated segment specification regulation of gene expression covalent chromatin modification protein sumoylation hemopoiesis negative regulation of gene expression, epigenetic positive regulation of fibroblast proliferation positive regulation of ubiquitin-protein transferase activity skeletal system development in utero embryonic development DNA methylation regulation of transcription, DNA-templated humoral immune response brain development cellular process chromatin modification histone acetylation histone ubiquitination rostrocaudal neural tube patterning positive regulation of B cell proliferation positive regulation of immature T cell proliferation in thymus somatic stem cell division embryonic skeletal system morphogenesis embryonic skeletal system development regulation of catalytic activity hematopoietic stem cell homeostasis negative regulation of apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BMI-1 Monoclonal Antibody (3E3)

BMI-1 Antibody (MA5-15919) in ICC/IF

Product Details

MA5-15919

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

in situ PLA (PLA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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