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FIGURE: 1 / 4
BOK Antibody (PA5-106615) in ICC/IF
Immunocytochemistry analysis of BOK in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with BOK polyclonal antibody (Product # PA5-106615) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106615
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BOK(Accession Q9UMX3), corresponding to amino acid residues M1-S49.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854283
Product Specific Information
Antibody detects endogenous levels of total BOK.
Target Information
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains all four BCL-2 like domains and is a pro-apoptotic BCL-2 protein identified in the ovary.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apoptosis activator Mtd; Bcl-2-like protein 9; Bcl-2-related ovarian killer protein; BCL2 related ovarian killer; Bcl2-L-9; BCL2-related ovarian killer; BCL2-related ovarian killer protein; hBOK; Protein matador
Gene Aliases: AI847676; AU021146; BCL2L9; BOK; Bok-BH3; BOKL; matador; Mtd
UniProt ID: (Human) Q9UMX3, (Rat) Q792S6, (Mouse) O35425
Entrez Gene ID: (Human) 666, (Rat) 29884, (Mouse) 51800
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
brain development
cell proliferation
male gonad development
intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of apoptotic process
oligodendrocyte differentiation
neuron apoptotic process
intrinsic apoptotic signaling pathway by p53 class mediator
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of execution phase of apoptosis
regulation of apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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