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BORIS Antibody (600-401-907) in WB
Western blot using Rocklands Affinity Purified anti-BORIS antibody shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead). Lane 1 contains lysate from human prostate tissue. Lane 2 contains lysate from human spleen tissue. A predominant band at ~75 kDa is observed. Molecular weight estimation was made by comparison to prestained MW markers as indicated.
Product Details
600-401-907
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chimpanzee,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-Terminal region near aa 1-30 of human BORIS protein.
Conjugate
Form
Liquid
Concentration
1.28 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against the human BORIS protein. A BLAST analysis was used to suggest cross reactivity with BORIS proteins from human and chimpanzee. Partial reactivity may occur against BORIS from mouse, dog and rat sources based on varying degrees of homology to the immunizing sequence. Reactivity against homologues from other sources is not known.
Target Information
CTCFL is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes. CTCFL is normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BORIS like protein; BORIS-like protein; Brother of the regulator of imprinted sites; Cancer/testis antigen 27; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein)-like; CT27; CTCF paralog; CTCF T; CTCF-like protein; HMG 1L1; HMG-1L1; MGC163358; MGC169105; MGC169106; putative high mobility group protein 1-like 1; putative high mobility group protein B1-like 1; Transcriptional repressor CTCFL; Zinc finger protein CTCF-T
Gene Aliases: BORIS; CT27; CTCF-T; CTCFL; dJ579F20.2; HMGB1L1
UniProt ID: (Human) Q8NI51
Entrez Gene ID: (Human) 140690
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
protein binding
histone binding
sequence-specific DNA binding
transcription regulatory region DNA binding
metal ion binding
regulation of gene expression by genetic imprinting
transcription from RNA polymerase II promoter
cell cycle
positive regulation of gene expression
histone methylation
DNA methylation involved in gamete generation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of histone H3-K4 methylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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