Proteintech

BRCA1 Polyclonal Antibody

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View all (78) BRCA1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

22362-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 19 publications 19 publications

Immunohistochemistry (IHC)

-
View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200
-

Immunocytochemistry (ICC/IF)

-
View 4 publications 4 publications

Immunoprecipitation (IP)

-
View 1 publication 1 publication

ChIP assay (ChIP)

-
View 1 publication 1 publication

Not applicable (N/A)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BRCA1 Fusion Protein Ag17946 (1514-1863 aa encoded by BC115037)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to BRCA1. BRCA1 appears to produce multiple splice variants. BRCA1 is a nuclear protein with a molecular mass of 220 kDa. BRCA1D672-4095 is generated by exclusion of exon 11 by in-frame splicing and produces a 97 kDa protein. In contrast to BRCA1, BRCA1D672-4095 localizes to the cytoplasm.

Immunogen sequence: SGSLQNRNY PSQEGLIKVV DVEEQQLEES GPHDLTETSY LPRQDLEGTP YLESGISLFS DDPESDPSED RAPESARVGN IPSSTSALKV PQLKVAESAQ GPAAAHTTDT AGYNAMEESV SREKPELTAS TERVNKRMSI VVSGLTPEEF MLVYKFARKH HITLTNLITE ETTHVVMKTD AEFVCERTLK YFLGIAGGKW VVSYFWVTQS IKERKMLNEH DFEVRGDVVN GRNHQGPKRA RESQDRKIFR GLEICCYGPF TNMPTDQLEW MVQLCGASVV KELSSFTLGT GVHPIVVVQP DAWTEDNGFH AIGQMCEAPV VTREWVLDSV ALYQCQELDT YLIPQIPHSH Y (1514-1863 aa encoded by BC115037)

Target Information

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: brca 1; brca-1; BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein 1; breast cancer 1; breast cancer 1 early onset; breast cancer 1, early onset; Breast cancer type 1 susceptibility protein; BROVCA12; Delta772-3095; early onset breast cancer 1; Fanconi anemia, complementation group S; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; protein phosphatase 1, regulatory subunit 53; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; truncated BRCA1; truncated breast and ovarian cancer susceptibility protein 1; truncated breast and ovarian cancer sususceptibility protein 1; truncated breast cancer type 1 susceptibility protein

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Gene Aliases: BRCA1; BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53

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UniProt ID: (Human) P38398

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Entrez Gene ID: (Human) 672

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Function(s)
DNA binding chromatin binding damaged DNA binding transcription coactivator activity RNA binding ubiquitin-protein transferase activity protein binding zinc ion binding tubulin binding ligase activity enzyme binding ubiquitin protein ligase binding transcription regulatory region DNA binding androgen receptor binding
Process(es)
double-strand break repair via homologous recombination DNA double-strand break processing DNA synthesis involved in DNA repair strand displacement DNA replication postreplication repair double-strand break repair double-strand break repair via nonhomologous end joining regulation of gene expression by genetic imprinting transcription, DNA-templated regulation of transcription from RNA polymerase II promoter regulation of transcription from RNA polymerase III promoter fatty acid biosynthetic process apoptotic process cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator chromosome segregation centrosome cycle brain development response to nutrient intrinsic apoptotic signaling pathway in response to DNA damage dosage compensation by inactivation of X chromosome response to ionizing radiation positive regulation of vascular endothelial growth factor production positive regulation of gene expression protein ubiquitination protein sumoylation androgen receptor signaling pathway chromosome breakage positive regulation of protein ubiquitination G2 DNA damage checkpoint response to estradiol negative regulation of intracellular estrogen receptor signaling pathway positive regulation of protein import into nucleus, translocation positive regulation of histone acetylation negative regulation of histone acetylation regulation of cell proliferation regulation of apoptotic process chordate embryonic development response to estrogen regulation of DNA methylation negative regulation of fatty acid biosynthetic process positive regulation of DNA repair positive regulation of angiogenesis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of centriole replication positive regulation of histone H3-K4 methylation negative regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation positive regulation of histone H3-K9 methylation protein autoubiquitination positive regulation of histone H4-K20 methylation positive regulation of cell cycle arrest cellular response to tumor necrosis factor cellular response to indole-3-methanol protein K6-linked ubiquitination regulation of signal transduction by p53 class mediator negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of reactive oxygen species metabolic process positive regulation of histone H3-K9 acetylation positive regulation of histone H4-K16 acetylation
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