Invitrogen

BRCA1 Monoclonal Antibody (8F7)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-23162

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8F7

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 341-748.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_557809

Product Specific Information

Recommended positive controls: 293T.

For immunohistochemistry (paraffin), perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1M citrate buffer at 95ºC in water bath for 20 minutes). Avoid nonspecific binding by incubation in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes.

Target Information

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: brca 1; brca-1; BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein 1; breast cancer 1; breast cancer 1 early onset; breast cancer 1, early onset; breast cancer associated 1; Breast cancer type 1 susceptibility protein; Breast cancer type 1 susceptibility protein homolog; BROVCA12; Delta772-3095; early onset breast cancer 1; Fanconi anemia, complementation group S; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; protein phosphatase 1, regulatory subunit 53; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; truncated BRCA1; truncated breast and ovarian cancer susceptibility protein 1; truncated breast and ovarian cancer sususceptibility protein 1; truncated breast cancer type 1 susceptibility protein

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Gene Aliases: BRCA1; BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53

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UniProt ID: (Human) P38398, (Mouse) P48754

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Entrez Gene ID: (Human) 672, (Mouse) 12189

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Function(s)
DNA binding chromatin binding damaged DNA binding transcription coactivator activity RNA binding ubiquitin-protein transferase activity protein binding zinc ion binding tubulin binding ligase activity enzyme binding ubiquitin protein ligase binding transcription regulatory region DNA binding androgen receptor binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
Process(es)
double-strand break repair via homologous recombination DNA replication DNA repair postreplication repair double-strand break repair DNA recombination regulation of gene expression by genetic imprinting lipid metabolic process fatty acid metabolic process fatty acid biosynthetic process cellular response to DNA damage stimulus cell cycle chromosome segregation centrosome cycle intrinsic apoptotic signaling pathway in response to DNA damage dosage compensation by inactivation of X chromosome response to ionizing radiation positive regulation of vascular endothelial growth factor production positive regulation of gene expression protein ubiquitination chromosome breakage positive regulation of protein ubiquitination G2 DNA damage checkpoint positive regulation of histone acetylation negative regulation of histone acetylation regulation of cell proliferation chordate embryonic development response to estrogen regulation of DNA methylation negative regulation of fatty acid biosynthetic process positive regulation of DNA repair positive regulation of angiogenesis negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter centrosome duplication positive regulation of histone H3-K4 methylation negative regulation of histone H3-K4 methylation negative regulation of histone H3-K9 methylation positive regulation of histone H3-K9 methylation protein autoubiquitination positive regulation of histone H4-K20 methylation positive regulation of cell cycle arrest cellular response to tumor necrosis factor cellular response to indole-3-methanol protein K6-linked ubiquitination negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of reactive oxygen species metabolic process positive regulation of histone H3-K9 acetylation positive regulation of histone H4-K16 acetylation DNA double-strand break processing DNA synthesis involved in DNA repair strand displacement double-strand break repair via nonhomologous end joining transcription, DNA-templated regulation of transcription from RNA polymerase II promoter regulation of transcription from RNA polymerase III promoter apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator brain development response to nutrient protein sumoylation androgen receptor signaling pathway response to estradiol negative regulation of intracellular estrogen receptor signaling pathway positive regulation of protein import into nucleus, translocation regulation of apoptotic process positive regulation of transcription, DNA-templated negative regulation of centriole replication regulation of signal transduction by p53 class mediator
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